| 黄瓜农杆菌介导法与花粉管通道法转基因技术 |
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| 引用本文： | 张文珠,魏爱民,杜胜利,韩毅科,张桂华,刘楠.黄瓜农杆菌介导法与花粉管通道法转基因技术[J].西北农业学报,2009,18(1):217-220. |
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| 作者姓名： | 张文珠 魏爱民 杜胜利 韩毅科 张桂华 刘楠 |
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| 作者单位： | 天津科润黄瓜研究所,天津,300192 |
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| 摘 要： | 采用农杆菌介导法和花粉管通道法进行抗虫基因EQKAM转入黄瓜的研究.优化了以子叶节为外植体的农杆菌介导转化技术;比较了切割柱头、子房注射和子房涂抹3种花粉管通道法处理授粉后黄瓜子房的转化效果,切割柱头和子房注射均可成功获得黄瓜抗虫转基因植株.PCR及斑点杂交证明EQKAM基因已整合到黄瓜基因组中.
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| 关 键 词： | 黄瓜 农杆菌 花粉管通道 转基因 |
| 收稿时间： | 2008/6/26 0:00:00 |
| 修稿时间： | 2008/7/28 0:00:00 |
Transformation Technology Through A. tumefaciens Mediation and Pollen Tube Pathway in Cucumber |
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| ZHANG Wenzhu,WEI Aimin,DU Shengli,HAN Yike,ZHANG Guihua and LIU Nan.Transformation Technology Through A. tumefaciens Mediation and Pollen Tube Pathway in Cucumber[J].Acta Agriculturae Boreali-occidentalis Sinica,2009,18(1):217-220. |
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| Authors: | ZHANG Wenzhu WEI Aimin DU Shengli HAN Yike ZHANG Guihua and LIU Nan |
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| Institution: | Tianjin Kernel Cucumber Research Institute, Tianjin 300192, China;Tianjin Kernel Cucumber Research Institute, Tianjin 300192, China;Tianjin Kernel Cucumber Research Institute, Tianjin 300192, China;Tianjin Kernel Cucumber Research Institute, Tianjin 300192, China;Tianjin Kernel Cucumber Research Institute, Tianjin 300192, China;Tianjin Kernel Cucumber Research Institute, Tianjin 300192, China |
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| Abstract: | Transformation of insect resistant gene EQ KAM to cucumber was studied. Medium for A.tumefaciens mediated transformation using cotyledonary node as explants was optimized;3 methods including cutting chapiter,ovary injection and chapiter spreading for pollen tube pathway was compared. Cutting chapiter and ovary injection can both achieve insect resistant gene tranformants. PCR and dot blot test indicated that gene EQKAM had been incorporated into cucumber genome. |
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| Keywords: | Cucumber(C sativus L ) A tumefaciens Pollen tube pathway Transformation |
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