不同毒力的猪轮状病毒感染仔猪后肠道病理学观察

用通过MA104细胞培养,经不同代次后,两种毒力不同的猪轮状病毒细胞毒,口服感染未吮初乳的1日龄仔猪,于29.5或48小时后宰杀,作消化道病理学及电镜超微结构变化的比较观察,结果证明,两种细胞毒,毒力虽不同,但都能侵入肠上皮细胞生长繁殖,而所引起的病理变化,则有不同。MA86F23细胞毒(强毒)能引起小肠段大量绒毛变形、脱落,绒毛固有层充血、出血、有嗜中性白细胞浸润,粘膜下水肿和充血等炎症反应。而口服MA86F90细胞毒(弱毒)的乳猪,其小肠绒毛大部分还保持完整,仅有少部分脱落,肠粘膜及肠腺分泌亢进,此外,在粘膜下见有多个淋巴细胞呈结节状增生。所设健康对照猪,基本无变化。

PATHOLOGIC CHANGES IN THE SMALL INTESTINE OF PIGLETS INFECTED WITH DIFFERENT VIRULENT STRAINS OF PORCINE ROTAVIRUS

Pathologic changes in the small intestine of piglets ( deprived from colostrum ) were studied under light and electron microscope, 29.5 and 48 hours after oral infection with two different vrrulent strains of porcine rotavirus. The two strains were propagated in A104 cell cultures of different passages (23th, MA86 F23 and 90th, MA86 F90). The viruses of both passage readily grow in the epithelial cell of small intestine. The pathologic changes were: loss of vili and inflamatory reactions in the small intestine, such as hyperemia, hemorrhage and infiltration of neutrophil leukocytes in the lamina propria. Edema and hyperemia of submucosa were also observed in newborn pigiets infected with MA86F23 rotavirus, while most of the vili in the small intestine remain normal with only a few vili loss and oversecretion of the mu-cosa and gland in the small intestine and lymphocyte proliferation in the submucosa were observed in those infected with MA86F90 rotavi rus.No pathologic changes were seen in the control group.