| 辽宁抚顺樟子松松材线虫分离与鉴定 |
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| 引用本文： | 于海英,吴昊,黄瑞芬,王蛟,张睿轩,宋玉双. 辽宁抚顺樟子松松材线虫分离与鉴定[J]. 中国森林病虫, 2020, 39(2): 6-10 |
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| 作者姓名： | 于海英 吴昊 黄瑞芬 王蛟 张睿轩 宋玉双 |
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| 作者单位： | 国家林业和草原局森林和草原病虫害防治总站,林业有害生物监测预警国家林业和草原局重点实验室,辽宁 沈阳 110034;辽宁省林业有害生物防治检疫工作站,辽宁 沈阳 110084;清原县苍石林场,辽宁 清原 113200 |
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| 基金项目： | 辽宁省重点研发计划“辽宁地区松材线虫自然传播规律及综合防控技术研究”(2019JH2/10200001);辽宁省重点研发指导计划“松材线虫内寄生真菌Esteya vermicola的生防机制及应用技术研究”(2019JH8/10200010)。 |
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| 摘 要： | 2019年4-7月在辽宁省抚顺市对樟子松Pinus sylvestris var.mongolica枯死、濒死树取样调查,发现树体内含有线虫,经形态学比较、测量以及核糖体DNA序列分析,鉴定为松材线虫Bursaphelenchus xylophilus。樟子松是欧洲赤松Pinus sylvestris的变种,我国早期的接种试验表明樟子松为人工接种感病树种,但始终未见樟子松自然感染现象,此文是我国自然条件下樟子松感染松材线虫的首次报道。
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| 关 键 词： | 樟子松 松材线虫 形态学 分子生物学 新寄主植物 |
Separation and identification of Bursaphelenchus xylophilus from Pinus sylvestris var. mongolica in Fushun city |
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| Affiliation: | (General Station of Forest and Grassland Pest Management,National Forestry and Grassland Administration,Key Laboratory of National Forestry and Grassland Administration on Forest Pest Monitoring and Warning,Shenyang 110034,China) |
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| Abstract: | From April to July in 2019,Pinus sylvestris var.mongolica of dead and dying trees in Fushun city,Liaoning province were sampled and investigated,and found to contain nematodes,which were identified as Bursaphelenchus xylophilus by morphological comparison,measurement,and ribosomal DNA sequence analysis.P.sylvestris var.mongolica is a variant of Pinus sylvestris.Early inoculation tests in China showed that P.sylvestris var.mongolica was an artificially inoculated susceptible species with B.xylophilus.However,there has not been a natural infection of pine wood nematode in P. sylvestris var.mongolica.This is the first report on P.sylvestris var.mongolica infected naturally by B.xylophilus in China. |
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| Keywords: | Pinus sylvestris var.mongolica Bursaphelenchus xylophilus morphology molecular biology new host plant |
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