洋葱槲皮素对鸡免疫器官指数、巨噬细胞吞噬功能及分泌细胞因子的影响

为了探讨洋葱槲皮素对鸡免疫功能的影响,研究选用180只健康1日龄海兰褐公鸡雏,设空白对照组、阳性对照组、1 g/kg洋葱槲皮素组、2 g/kg洋葱槲皮素组、4 g/kg洋葱槲皮素组和8 g/kg洋葱槲皮素组。每组30只鸡雏,自由采食、饮水。1日龄开始,阳性对照组在饮水中按0.05 g/L加入左旋咪唑,1 g/kg洋葱槲皮素组、2 g/kg洋葱槲皮素组、4 g/kg洋葱槲皮素组和8 g/kg洋葱槲皮素组在饲料中分别按1、2、4、8 g/kg加入洋葱槲皮素,连喂28 d。在29和36日龄,采用称重法检测洋葱槲皮素对鸡脾、法氏囊和胸腺指数的影响;在29日龄,采用MTT法检测洋葱槲皮素对鸡腹腔巨噬细胞吞噬功能的影响;采用ELISA法检测洋葱槲皮素对鸡腹腔巨噬细胞分泌IL-4和IL-12的影响。结果显示,洋葱槲皮素能够提高脾、法氏囊和胸腺指数;提高巨噬细胞吞噬功能,诱导巨噬细胞IL-4和IL-12的分泌量。4 g/kg洋葱槲皮素组的作用效果优于1 g/kg洋葱槲皮素组、2 g/kg洋葱槲皮素组和8 g/kg洋葱槲皮素组,呈双向调节。试验表明,洋葱槲皮素可通过促进脾、法氏囊和胸腺的发育,提高巨噬细胞的吞噬功能,诱导巨噬细胞IL-4和IL-12分泌,进而提高机体的免疫功能。

Effect of onion quercetin on immune organ index,phagocytic function of macrophages and secretion cytokine in chickens

In order to investigate the effect of onion quercetin on chicken immune function, 180 healthy one-day-old Hailan Brown cocks were divided into blank control group, positive control group, 1 g/kg onion quercetin group, 2 g/kg onion quercetin group, 4 g/kg onion quercetin group and 8 g/kg onion quercetin group. Thirty chickens in each group were fed and drinked freely. From the first day of age, the positive control group was added with levamisole at 0.05 g/L in drinking water, 1 g/kg onion quercetin group, 2 g/kg onion quercetin group, 4 g/kg onion quercetin group and 8 g/kg onion quercetin group were fed respectively with onion quercetin at 1 g/kg, 2 g/kg, 4 g/kg and 8 g/kg for 28 days. At the age of 29 and 36 days, the effect of onion quercetin on chicken spleen, bursa of fabricius and thymus index were measured by weighing method;At the age of 29 days, the effect of onion quercetin on the phagocytic function of chicken peritoneal macrophages was detected by MTT method;The effects of onion quercetin on the secretion of IL-4 and IL-12 from chicken peritoneal macrophages were detected by ELISA. Onion quercetin could improve spleen, bursa of fabricius and thymus index. It can enhance the phagocytic function of macrophages and induce the secretion of IL-4 and IL-12 in macrophages. The effect of 4 g/kg quercetin group was better than that of 1 g/kg quercetin group, 2 g/kg quercetin group and 8 g/kg quercetin group, showing bidirectional regulation. The results showed that onion quercetin could promote the development of spleen, bursa of fabricius and thymus, improve the phagocytic function of macrophages, and induce the secretion of IL-4 and IL-12 of macrophages, so as to improve the immune function of the body.