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HPLC和比色法测定鸭蛋蛋黄粉中胆固醇含量的比较
引用本文:黄红霞,张晓斌,张声华.HPLC和比色法测定鸭蛋蛋黄粉中胆固醇含量的比较[J].湖北农业科学,2009,48(2).
作者姓名:黄红霞  张晓斌  张声华
作者单位:1. 湖北工业大学生物工程学院,武汉,430068
2. 华中农业大学食品科技学院功能食品与分子生物学实验室,武汉,430070
摘    要:试验通过直接皂化-比色法和直接皂化-HPLC两种方法测定了鸭蛋蛋黄粉中胆固醇的含量.结果表明,这两种方法测定的胆固醇的含量有一定的差异.用比色法测定的蛋品中胆固醇的含量要高于用高效液相色谱法测定的胆固醇的含量.

关 键 词:鸭蛋蛋黄  胆固醇  比色法

Cholesterol Analysis in Duck's Eggs by Direct Saponification-colorimetric and HPLC Methods
HUANG Hong-xia,ZHANG Xiao-bin,ZHANG Sheng-hua.Cholesterol Analysis in Duck's Eggs by Direct Saponification-colorimetric and HPLC Methods[J].Hubei Agricultural Sciences,2009,48(2).
Authors:HUANG Hong-xia  ZHANG Xiao-bin  ZHANG Sheng-hua
Institution:1.College of Biological Engineering;Hubei University of Technology;Wuhan 430068;China;2.Laboratory of Functional Food and Molecular Biology bases;Food Science and Technology College;Huazhong Agricultural University;Wuhan 430070;China
Abstract:The cholesterol in powder of duck egg yolk was measured by HPLC and colorimetry method after direct saponification in this paper. The results indicated that some differences existed in content of cholesterol. The content of cholesterol by colorimetric method was higher than HPLC method.
Keywords:HPLC
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