| 蚕豆种子离体萌发和快繁技术研究 |
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| 作者姓名： | 屈巧玲 胡润田 康诗腾 许凌波 王亦菲 陆瑞菊 |
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| 作者单位： | 上海市文来中学 上海201101(屈巧玲，胡润田，康诗腾，许凌波)，上海市农业科学院生物技术研究中心 上海市农业遗传育种重点实验室上海201106(王亦菲，陆瑞菊) |
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| 摘 要： | 以蚕豆为研究对象,比较了种子处理对种子发芽率的影响、培养基中添加AC,Vc和PVP对抑制外植体褐化的效果以及培养基中添加MET、香蕉、椰汁和马铃薯对试管苗繁殖系数及玻璃苗的影响。结果表明发芽时保留种皮、接种时胚根向上对蚕豆种子的萌发有着至关重要的影响,用5.0mg/LGA3溶液浸种12h有利于种子萌发;培养基中添加0.5g/LAC能有效抑制外植体的褐化,并能使植株生长健壮;培养基中添加150mg/L椰汁能提高繁殖系数,有效抑制玻璃苗的发生。
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| 关 键 词： | 蚕豆 快繁 繁殖系数 玻璃苗 |
Study on invitro of Germination and Rapid Propagation of Vicia faba L. |
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| Authors: | Qu Qiaoling Hu Runtian Kang Shiteng Xu Lingbo Wang Yifei Lu Ruiju |
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| Institution: | 1.Shanghai Wenlai middle school, Shanghai 201101; 2.Biotech Research Center, Shanghai Academy of Agricultural Sciences; 3.Shanghai Key Lab. of Agricultural Genetics and Breeding, Shanghai 201106 |
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| Abstract: | Using Vicia faba as donor materials, the effects of seed-treatment on germination, supplementing AC, Vc and PVP to induction media on explant-browning and adding MET, banana, potato and coconut water to propagating media on propagating coefficient and bud-vitrifiation were investigated. The results showed that the frequency of germination were able to be increased by keeping seed vessel, culturing seeds with radicle up and pre-treating seeds in 5.0mg/L GA3 solution for 12h. The frequency of explant-browning was reduced by adding AC 0.5mg/L to induction media and the vigor of plantlets was promoted by the AC-adding. The propagating coefficient could be improved and the frequency of bud-vitrifration increased by adding 150mg/L coconut water. |
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| Keywords: | Vicia faba L Rapid propagation Propagation coefficient Vitreous buds |
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