| 不同培养条件下长寿花叶片再生体系的构建 |
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| 作者姓名： | hnnxflj@.com |
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| 作者单位： | 河南农业职业学院植物科学系 河南中牟451450 |
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| 摘 要： | 以长寿花粉红色品种离体叶片为外植体,探讨了基本培养基、暗培养以及不同植物生长调节剂组合对不定芽再生的影响。结果表明,基本培养基以MS最为合适,以1/2MS BA2.0mg/L NAA0.1mg/L可以使粉红色品种的叶片不定芽的再生率高达5.2,暗培养15天可以将叶片的不定芽再生率提高到5.47。生根培养用1/2MS IBA0.1mg/L最好。
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| 关 键 词： | 白花长寿花 组织培养 快速繁殖 |
Research of Different Culture Conditions on Plantlet Regeneration from Leaves of Kalanchoe blossfeldiana |
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| Authors: | S Q |
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| Institution: | Henan Vocational College of Agriculture, zhongmu henan 451450 |
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| Abstract: | The ideal regeneration system of Kalanchoe blossfeldiana with pink flower by leaf explants was established in this study, and the effects of basic medium, dark periods and plant growth regulators external on shoot regeneration from leaves of Kalanchoe blossfeldiana were investigated. The results showed that the highest tratious on regeneration rate of 5.20 was obtained on the medium MS supplemented with 2.0 mg/L BA and 0.5 mg/L NAA .Two weeks of dark treatment could increase the shoot regeneration rate to 5.47. MS supplemented with0.1mg/l IBA for rooting. |
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| Keywords: | Kalanchoe blossfeldiana Tissue culture Propagation |
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