| 虾青素合成关键酶基因bkt植物表达载体的构建及对玉米的遗传转化 |
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| 引用本文： | 徐 静,曲延英,杨庆利,禹山林,檀琮萍,侯艳华,秦 松. 虾青素合成关键酶基因bkt植物表达载体的构建及对玉米的遗传转化[J]. 勤云标准版测试, 2006, 0(8) |
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| 作者姓名： | 徐 静 曲延英 杨庆利 禹山林 檀琮萍 侯艳华 秦 松 |
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| 作者单位： | 新疆农业大学农学院 乌鲁木齐830052(徐静，曲延英)，中国科学院海洋研究所 青岛266071(杨庆利，檀琮萍，侯艳华，秦松)，山东省花生研究所 青岛266100(禹山林) |
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| 基金项目： | 国家“863”项目（2004AA241150）和青岛市自然科学基金项目（05-1-JC-92） |
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| 摘 要： | 虾青素是一种具有极强抗氧化活性的类胡萝卜素,具有广泛的应用价值。β-胡萝卜素酮化酶(Bkt)是由玉米黄素合成虾青素生物合成途径中的关键酶。采用LaTaqDNA聚合酶用PCR的方法从pET-28a( )bkt中扩增得到bkt基因,用bkt基因替换pBI221中的GUS基因形成含有CaMV35S启动子和NOS终止子的bkt基因表达盒,然后插入植物表达载体pCAMBIA1301的多克隆位点,最终获得带有选择标记和报告基因的植物表达载体pCAMBIA1301-bkt。通过农杆菌LBA4404介导将其转化进入玉米自交系齐319,转化后的愈伤经GUS组织化学染色分析表明bkt基因已经转入玉米胚性愈伤组织。
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| 关 键 词： | 虾青素 bkt基因 载体构建 玉米胚性愈伤 转化 |
Construction of Plant Expression Vector Containing Genes Encoding The Key Biosynthesis Enzyme of Astaxanthin( bkt) and Its Expression in Zea mays L. |
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| Xu jing,Qu Yanying,Yang Qinli,Yu shanlin,Tan Congping,Hou Yanhu,Qin song. Construction of Plant Expression Vector Containing Genes Encoding The Key Biosynthesis Enzyme of Astaxanthin( bkt) and Its Expression in Zea mays L.[J]. , 2006, 0(8) |
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| Authors: | Xu jing Qu Yanying Yang Qinli Yu shanlin Tan Congping Hou Yanhu Qin song |
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| Affiliation: | 1.Agricultural College, Xinjiang Agricultural University,Urumqi 830052;2.Institute of Oceanology, Chinese Academy of Sciences,Qingdao 266071; 3.Shandong Peanut Research lnstitute,Qingdao 266100 |
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| Abstract: | Astaxanthin with high antioxidant activity is of great application value. Bkt is the key biosynthesis enzyme of astaxanthin which encoded by bkt gene. Bkt gene expression cassette was constructed by replacing GUS gene in vector pBI221 with bkt gene obtained by PCR method, which contained the CaMV 35S promoter and NOS terminator. Plant expression vector pCAMBIA1301-bkt was constructed by inserting bkt gene expression cassette into multiple cloning site in vector pCAMBIA1301.Bkt gene was transferred into maize Qi 319 mediated by Agrobacterium tumefaciens. The results of GUS histochemical assay indicated that bkt gene had been transferred into maize emberyogenic calli. |
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| Keywords: | astaxanthin bkt plant expression vector maize emberyogenic calli transformation |
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