| 不同处理方法对家兔超数排卵效果的影响 |
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| 作者姓名： | 林 峰 陈玉霞 史明艳 高腾云 |
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| 作者单位： | 河南农业大学牧医工程学院 郑州450002(林峰，陈玉霞，高腾云)，西北农林科技大学动物科技学院 陕西杨凌712100(史明艳) |
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| 基金项目： | 河南省重大科技攻关项目（0422011200）资助. |
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| 摘 要： | 采用两种处理方法对家兔进行了超数排卵研究。结果表明:PMSG-HCG法处理的母兔卵巢上的平均排卵点数极显著(P<0.01)高于PMSG法,而PMSG-HCG法的平均囊肿卵泡数与平均卵泡数则显著(P<0.05)低于PMSG法;两种方法间的平均卵泡发育数则差异不显著(P>0.05)。说明PMSG-HCG法的超排效果明显优于PMSG法。试验还表明,母兔交配后28h采胚所获取的胚胎大多处于二细胞期,到交配后32h,胚胎多处于四细胞期。采用PMSG-HCG法进行超排处理,对家兔的胚胎发育无影响。
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| 关 键 词： | 家兔 超数排卵 胚胎发育 |
Effect on Superovulation of Rabbit Using Different Methods |
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| Authors: | Lin Feng Chen Yuxi Shi Mingyan Gao Tengyun |
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| Institution: | 1.College of Animal Husbandry and Veterinary, Henan Agricultural University, Zhengzhou 450002; 2.College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100 |
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| Abstract: | Two methods were applied to research the superovulation of Rabbits. The results showed that the average number of the ovulation points on the ovaries of the rabbits using PMSG-HCG method was extremely different (P<0.01) from those of PMSG method, and the average number of the cystic follicles and follicles of PMSG-HCG method was significant difference (P<0.05) from that of PMSG method. The average number of the developed follicles of the two methods was not significant difference (P>0.05). The superovulated effect of PMSG-HCG method was significantly better than that of PMSG method. The results also showed that most of the gained embryos were in 2-cell stage during 28 hours after the rabbits accepted mating, and in 4-cell stage during 32 hours after the rabbits accepted mating. PMSG-HCG method didn''t influence upon the development of the rabbits'' embryos when it was applied to study on their superovulation effect. |
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| Keywords: | Rabbit Superovulation Embryo development |
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