| 农杆菌介导的抗菌肽基因SPCEMA对马铃薯的遗传转化 |
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| 引用本文： | limy@swau.cq.cn. 农杆菌介导的抗菌肽基因SPCEMA对马铃薯的遗传转化[J]. 勤云标准版测试, 2006, 0(5) |
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| 作者姓名： | limy@swau.cq.cn |
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| 作者单位： | 西南大学园艺园林学院 重庆400716(杨利荣,李名扬,祝钦泷),西南大学农业部蚕桑学重点实验室 重庆400716(徐立) |
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| 基金项目： | 重庆市科委生物技术攻关项目“马铃薯抗晚疫病的基因工程”资助[批准文号：渝科计（1998）18]. |
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| 摘 要： | 为了得到马铃薯抗病材料,本研究利用根癌农杆菌(Agrobacteriumtumefaciens)LBA4404转化马铃薯栽培品种“台湾红”,建立了优良的遗传转化系统:根癌农杆菌浓度以D600nm=0.5￣0.8为宜,感染时间以5min为宜;茎段和叶盘的预培养时间分别以4d和8d为宜,共培养时间皆以3d为宜。通过PCR和Sourhernblotting检测证明抗菌肽基因SPCEMA已成功导入其中。其茎段和叶片为的转化频率分别为10%和5.79%。
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| 关 键 词： | 马铃薯 根癌农杆菌 遗传转化 SPCEMA基因 |
The Introduction of SPCEMA Gene into Potato (solanum tuberosum L.) by Agrobacterium tumefaciens |
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| Yang Lirong,Li Mingyang,Zhu Qinlong,Xu Li. The Introduction of SPCEMA Gene into Potato (solanum tuberosum L.) by Agrobacterium tumefaciens[J]. , 2006, 0(5) |
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| Authors: | Yang Lirong Li Mingyang Zhu Qinlong Xu Li |
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| Affiliation: | 1 College of Horticulture and Landscape Engineering, Southwest University, Chongqing 400716; 2 The Key Sericultural Laboratory of Agriculture of Ministry, Southwest University, Chongqing 400716 |
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| Abstract: | In order to search an antibacterial potato (Solanum tuberosum L.), Various conditions for potato transformation with Agrobacterium tumefaciens LBA4404 were investigated and optimal conditions for the cultivar " Taiwanhong" were founded. At the same time, an efficient transformation system was developed. 1)The D600 of the Agrobacterium suspension was adjusted to 0.5~0.8 and then stems and leaf discs were dropped into the suspension for 5 min. 2)Stems and leaves were pre-cultured for 4 days and 8 days respectively. 3)The duration of co-culture is 3 days. Through this system SPCEMA gene was introduced into "Taiwanhong" and this was confirmed by PCR and Southern blotting. The transformation frequency of the stems and leaves was 10.00% and 5.79% respectively. |
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| Keywords: | Potato Agrobacterium tumefaciens Transformation SPCEMA |
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