猪MAPK12基因cDNA的克隆及序列分析

目的克隆新的猪MAPK12基因全长ORF,分析其生物特性,为进一步研究该酶的功能提供信息资料。方法:以已报道的人及小鼠MAPK12基因cDNA序列为依据,利用电脑克隆策略获得的ESTs设计引物,RT-PCR技术扩增新的猪MAPK12基因ORF序列,将PCR产物直接进行序列测定。分析此蛋白的特性并预测其结构。结果分离的ORF全长1101bp,编码367个氨基酸,与人鼠氨基酸序列92%同源;利用生物信息学软件分析出此蛋白的理化特性并预测了其一级、二级和高级结构。结论研究分离的基因片段可命名为猪ERK6,通过分析,获取了该酶的基本信息特征,并与现有的报道结果进行对比分析,为进一步开展猪MAPK12基因的结构功能、表达调控的相关研究奠定了基础。

Cloning and Sequence Analysis of MAPK12 Gene cDNA in Pig

The biologic characters of pig were analyzed to acquire a new ERK6 gene in pig and provide information for its further study. Based on MAPK12 gene cDNA sequence of human and mouse, three pairs of primers were designed to amplify ORF of pig MAPK12 gene. The amino acid sequence was analyzed by bioinformatic methods and software. This ORF included 1101bp, encoding 367 amino acids, 92% similar to human and mouse. Its physical and chemical characters were analyzed and its primary and secondary structure was predicted. This isolate is named as pig ERK6 gene. Obtain of the enzyme''s basic information and contrast analysis of existing reports will play an important role on further study of its structure and function.