| 直接诱导不定芽的矮牵牛再生体系的建立 |
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| 作者姓名： | 陶妹英 贾彩红 徐碧玉 金志强 |
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| 作者单位： | 中国热带农业科学院热带作物生物技术国家重点实验室 海口571101 |
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| 基金项目： | 中国热带农业科学院基金资助项目“百合T-DNA插入突变库的建立和筛选”(编号:Rky0529)。 |
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| 摘 要： | 以矮牵牛的叶片作为外植体,研究了不同浓度的激素配比对叶片直接诱导不定芽、诱导愈伤组织分化不定芽及生根的影响。得到了最适的直接诱导不定芽培养基的激素配6-BA1.5mg/L IBA0.5mg/L、诱导愈伤组织分化不定芽的培养基的激素配比6-BA1.5mg/L NAA0.3～0.5mg/L及生根培养基IBA0.03～0.05mg/L。并在时间上,从芽的长势、芽的生根情况进行比较,结果表明:直接诱导的不定芽比诱导愈伤组织分化的不定芽所需的时间短、长势好、生根快。
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| 关 键 词： | 矮牵牛 再生体系 不定芽 |
Establishment of A Regeneration System of Petunia Hybrida |
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| Authors: | Tao Meiying Jia Caihong Xu Biyu Jin Zhiqiang |
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| Institution: | The State Key Laboratory of Tropical Crops Biotechnology, The Chinese Academy of Tropical Agricultural Sciences, Haikou 571101 |
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| Abstract: | The effects of different combination of hormone concentration on buds induction directly from leaf and calli, and shoots rooting are studied. The results indicated that the medium with 6-BA1.5mg/L IBA0.5mg/L was the most suitable for buds induction directly from leaf, the medium with 6-BA1.5mg/L NAA0.3~0.5mg/L for buds induction from calli, and the medium with IBA0.03~0.05mg/L was suitable for rooting. In addition, the time of buds induction directly from leaf is shorter than those from calli. |
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| Keywords: | Petunia hybrida Regeneration system Adventitious shoots |
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