甘蓝型油菜中油杂8号种子纯度的SSR鉴定

细胞质雄性不育三系杂交种的纯度鉴定是油菜种子生产中重要的一环。选用173对SSR引物在中油杂8号的亲本间扩增,从中筛选到6对扩增效果好、条带清晰且差异片断在亲本间能够重复出现的引物,其中P051、P078和P203三对SSR引物在杂种F1中同时表现出父母本的特征条带。用这3对引物对32份中油杂8号的DNA进行鉴定,发现P078和P203的鉴定结果完全相同,因此采用其中的P078引物对经过田间种植鉴定的中油杂8号样品进行了鉴定。结果表明,SSR鉴定的结果与田间种植鉴定的结果非常接近,说明采用P078或P203都可以对中油杂8号进行快速准确的鉴定。

Identification of Seed Purity of Brassica napus Zhongyouza No.8 using SSR markers

Purity identification for cytoplasm male sterility three-line hybrid seeds is very important in oilseed rape seed production. One hundred and seventy-three pairs of SSR primers were screened for polymorphic amplification between the parental lines of Zhongyouza No.8. Among those primers, six pairs amplified clear bands specific to either of the parents, with no difference among individual plants within lines. Three pairs of the primers, P051, P078 and P203, amplified all specific bands of both parents in F1 hybrid plants. Using the three primers, 32 DNA samples from Zhongyouza No.8 single plants were analyzed. P078 and P203 displayed the same result on seed purity. P078 was used to analyze a Zhongyouza No.8 seed sample whose purity had been identified in field. The result showed that the seed purity identified by SSR markers was very similar to field observation, indicating SSR primer pairs P078 and P203 can be used for quick and accurate identification of seed purity of Zhongyouza No.8.