| 鲢鳙打印病豚鼠气单胞菌主要特性及系统发育学分析 |
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| 引用本文： | chencuizhen@.com. 鲢鳙打印病豚鼠气单胞菌主要特性及系统发育学分析[J]. 勤云标准版测试, 2006, 0(5) |
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| 作者姓名： | chencuizhen@.com |
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| 作者单位： | 河北科技师范学院动物科学系 秦皇岛066600 |
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| 基金项目： | 河北省教育厅科技攻关项目 |
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| 摘 要： | 对从鲢(Hypophthalmichthysmolitrix)鳙(Aristichthysnobilis)打印病病死鱼中分离到的豚鼠气单胞菌(Aeromonascaviae),进行了形态与理化特性等方面较系统的表观分类学指征鉴定。同时择代表菌株(HC960704-1)进行了16SrRNA基因的分子鉴定,测定了16SrRNA基因序列、分析了相关细菌相应序列的同源性,构建了系统发生树;HC960704-1的16SrRNA基因序列长度为1416bp(GenBank登录号:AY966888),与GenBank数据库中的气单胞菌16SrRNA基因序列的相似性在98%和100%。
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| 关 键 词： | 鲢鱼 鳙鱼 打印病 豚鼠气单胞菌 主要特性 系统发育学 |
Biological Characteristics and Phylogenetic Analysis of Aeromonas Caviae Isolated from Hypophthalmichthys molitrix and Aristichthys nobilis |
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| S .. Biological Characteristics and Phylogenetic Analysis of Aeromonas Caviae Isolated from Hypophthalmichthys molitrix and Aristichthys nobilis[J]. , 2006, 0(5) |
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| Authors: | S . |
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| Affiliation: | Department of A nimal Science, Hebei Normal University of Science and Technology, Qinhuangdao, 066600 |
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| Abstract: | Identification of extensive phenotypic informations of Aeromonas caviae which were isolated from Hypophthalmichthys molitrix and Aristichthys nobilis were conducted, including morphological, physiological and biochemical characteristics. In addition, the molecular identification of representative strain (HC960704-1) were conducted, the 16S rRNA gene were sequenced and compared with that of related strains, molecular phylogenetic tree was constructed; the length of the 16S rRNA gene sequence of representative strain(HC960704-1) is 1416 bp (Database accession numbers in NCBI: AY966888), and the 16S rRNA gene sequence of representative strain (HC960704-1)exhibited high similarity (98% and 100%) with the 16S rRNA gene of Aeromonas from GenBank database. |
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| Keywords: | Hypophthalmichthys molitrix Aristichthys nobilis Stigmatosis A.caviae Characterization Phylogenetic analysis |
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