部分矮牵牛品种亲缘关系的SRAP分析

本文首次利用分子标记SRAP(sequence-related amplified polymorphism)对部分矮牵牛品种进行亲缘关系分析。采用88对引物对我国栽培的58个矮牵牛品种进行扩增，从中筛选得到20个多态性较高的引物组合，共产生389条多态性条带，平均每个引物组合产生19.5个多态性条带，每个引物组合分别产生13～40个扩增带，Jaccard's相似系数在0.55～0.87之间。在遗传距离(GD)为0.67处，可将58个矮牵牛品种分为4个类群，第一类群主要为暖色系列的品种，第二类群为黄色花品种，第三、四类群为冷色花品种，白色花品种聚类较散。本研究结果为开展矮牵牛新品种保护、种子纯度检测以及分子育种提供了分子生物学资料。

Genetic Relationship of Some Cultivars of Petunia hybrids Using SRAP Marker

In this paper, the molecular marker system-SRAP (sequence-related amplified polymorphism) was firstly applied on 58 Petunia hybrids' genetic relationship research, the results obtained was as follows: 20 primer pairs selected from 88 primer pairs amplified 389 polymorphic bands with an average of 19.5 polymorphic bands per primer pair, and the number of bands amplified by each primer pair was ranged from 13 to 40. Moreover, Jaccard's similarity coefficient ranged from 0.55 to 0.87. 58 varieties could be divided into 4 groups when the genetic distance was 0.67. The first group mainly included warm colour serieses, and yellow flower variety was in the second group, while cold colour varieties were clustered in the third and forth group. In addition, the white colour varieties were clustered in a wide range. Study on genetic relationship of Petunia is benefit for variety protection, seed purity testing and genetic breeding.