| 长沙地区奶牛无浆体动物感染试验及其传播途径调查 |
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| 引用本文： | 何德肆,李海辉,沈萍,欧阳叙向,胡述光,刘毅.长沙地区奶牛无浆体动物感染试验及其传播途径调查[J].动物医学进展,2009,30(6). |
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| 作者姓名： | 何德肆 李海辉 沈萍 欧阳叙向 胡述光 刘毅 |
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| 作者单位： | 1. 湖南生物机电职业技术学院,湖南长沙,410127
2. 湖南农业大学动物医学院,湖南长沙,410128 |
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| 摘 要： | 采集长沙地区某奶牛场疑似患病牛鲜血及牛舍周围吸血类昆虫(牛虻、厩蝇和蚊子等),分别作镜检观察,腹腔接种至试验小鼠体内(0.5 mL/只)进行培养和观察.为进一步证明其传播途径,将12只试验兔分别放置于牛舍不同方位饲养1周,进行临床感染试验.结果表明,病牛鲜血涂片染色后在血液红细胞内观察到呈圆点状或球状的无浆体虫体,绝大部分位于红细胞边缘,少数位于中央,染虫率达30%,患牛抗凝血在试验小鼠体内进行培养后,第4天开始在小鼠红细胞内查到虫体,第17天达到感染高峰;吸血类昆虫血液涂片、染色后,其中385片在血液红细胞内观察到虫体,虫体查出率达77%;吸血昆虫内容物在试验小鼠体内的培养情况大致与患牛抗凝血情况相同;在临床感染1周后的第6天,7只兔在血液红细胞中查到虫体,兔感染率达75%.
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| 关 键 词： | 无浆体 吸血类昆虫 动物接种 体内培养 感染率 |
Investigation on Experimental Infection with Anaplasma from Cows and the Transmission Route of Pathogen in Changsha of Hunan |
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| HE De-si,LI Hai-hui,SHEN Ping,OUYANG Xu-xiang,HU Shu-guang,LIU Yi.Investigation on Experimental Infection with Anaplasma from Cows and the Transmission Route of Pathogen in Changsha of Hunan[J].Progress In Veterinary Medicine,2009,30(6). |
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| Authors: | HE De-si LI Hai-hui SHEN Ping OUYANG Xu-xiang HU Shu-guang LIU Yi |
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| Institution: | HE De-si1,LI Hai-hui2,SHEN Ping1,OUYANG Xu-xiang1,HU Shu-guang1,LIU Yi2(1.Hunan Biological And Electromechanical College,Changsha,Hunan,410127,China,2.College of Veterinary Medicine,Hunan Agricultural University,410128,China) |
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| Abstract: | The blood of suspected infected cows and blood-sucking insects around the cowshed,which were collected from one dairy cow farm in Changsha,were examined by microscope or were cultivated and observed via intra-abdominal inoculation with the anticoagulated blood from infected cows(0.5 mL/mouse) in laboratory,respectively.Moreover,to determine the route of transmission,12 rabbits were examined in the clinical infection after cultivating for one week in the different aspects of the cowsheds.The results showed t... |
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| Keywords: | Anaplasma blood-sucking insects animal inoculation in vivo culture infection rate |
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