| 花叶矮牵牛嵌合体再生体系的建立 |
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| 引用本文： | 吕秀立,张冬梅,钱又宇.花叶矮牵牛嵌合体再生体系的建立[J].上海农业学报,2007,23(1):34-38. |
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| 作者姓名： | 吕秀立 张冬梅 钱又宇 |
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| 作者单位： | 1. 上海市园林科学研究所,上海 200232
2. 上海城投绿化科技发展有限公司,上海 200232 |
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| 基金项目： | 上海市绿化管理局(林业局)项目 |
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| 摘 要： | 以花叶矮牵牛为材料,研究了外植体、激素浓度、光照强度、叶片生理状态、暗培养时间等因素对诱导嵌合体不定芽的影响,建立了花叶矮牵牛嵌合体再生体系。结果表明:无菌苗在2000 lx光照条件下,置于MS 6-BA 0.2 mg/L培养基上,嵌合体诱导率最大达到90.2%;生理年龄为20 d的叶片,在MS 6-BA 0.4 mg/L IBA0.01 mg/L培养基中,嵌合体诱导率最大达到34.8%,暗培养会降低嵌合体的诱导率。
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| 关 键 词： | 花叶矮牵牛 嵌合体 再生体系 |
| 文章编号： | 1000-3924（2007）01-34-05 |
| 修稿时间： | 2005-12-272007-01-11 |
Establishment of plant chimera regeneration system in Petunia hybrida Vilm |
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| L Xiu-li,ZHANG Dong-mei,QIAN You-yu.Establishment of plant chimera regeneration system in Petunia hybrida Vilm[J].Acta Agriculturae Shanghai,2007,23(1):34-38. |
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| Authors: | L Xiu-li ZHANG Dong-mei QIAN You-yu |
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| Institution: | 1 Shanghai Landscape Gardening Research Institute, Shanghai 200232, China ; 2 Shanghai Chengtou Landscaping Technology Development Co. , Ltd. , Shanghai 200232, China |
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| Abstract: | The plants of Petunia hybrida were used as a testmaterial,and the effects of different explants,hormone concentrations,illumination conditions,explants' physiological conditions,and dark-culture time on inducing chimera buds were studied.The experimental results showed that the inductivity of chimera buds reached the maximum,or 90.2% when the germ-free plantlets were on the medium of MS 0.2 mg/L 6-BA and under light treatment of 2 000 lx.The leaves at physiological age of 20 days on the medium of MS 0.4 mg/L 6-BA 0.01 mg/L IBA reached the maximum inductivity of chimera buds,or 34.8%.And dark culture reduced the inductivity of chimera buds. |
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| Keywords: | Petunia hybrida Vilm Plant chimera Regeneration system |
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