| 农杆菌介导小白菜的gus基因瞬间表达 |
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| 引用本文： | 苏军,段榕琦,胡昌泉,王锋.农杆菌介导小白菜的gus基因瞬间表达[J].福建果树,2000(2):3-5. |
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| 作者姓名： | 苏军 段榕琦 胡昌泉 王锋 |
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| 作者单位： | 福建农科院农业遗传工程重点实验室,福州,350003 |
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| 基金项目： | 福建省自然科学基金资助项目 |
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| 摘 要： | 用组织化学定位法检测小白菜ｇｕｓ基因瞬间表达情况，分析三种农杆菌对小白菜的侵染能力，结果表明：ＡＧＬ－１对小白菜的侵染能力最强，ＥＨＡ１０５次之，ＬＢＡ４４０４最弱；通过培养后定时测定ｇｕｓ基因瞬间表达情况，确定农杆菌与小白菜子叶的最佳共培养时间２－３ｄ。
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| 关 键 词： | 小白菜 农杆菌 瞬间表达 遗传转化 gus基因 |
TRANSIENT EXPRESSION OF GUS GENE IN CHINESE CABBAGE TRANSFORMATIED BY AGROBECTERIUM TUMEFACIENS |
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| Su Jun,Duang Rongqi,Hu Chanquang,Wang Feng.TRANSIENT EXPRESSION OF GUS GENE IN CHINESE CABBAGE TRANSFORMATIED BY AGROBECTERIUM TUMEFACIENS[J].Fujian Fruits,2000(2):3-5. |
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| Authors: | Su Jun Duang Rongqi Hu Chanquang Wang Feng |
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| Abstract: | The cotyledons of Brassica chinensis were inoculated with 3 agrobacterium tumefaciens strains(AGL I,EHA105 and LBA4404)in combination with pBin19 derived binary plasmid p35SGUSINT,whitch carrying the NosnptII selection gene.and 35Sgus(intron)report gene.The result showed AGL 1 infected explants had the highist GUS ecpression,while the least GUS activeity were the explants infected with LBA4404.2 or 3days cocultivating were suitable for B.Chinensis cotyledons. |
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| Keywords: | Chinese cabbage cotyledons Agrobacterium tumefaciens GUS |
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