禽蛋中泰乐菌素残留量的微生物学检测方法

试验建立了泰乐菌素在禽蛋残留中微生物学检测方法,将试样均质1min(5000r/min)后,称取20g置于离心管内,加入5ml水,充分搅匀,放置60min后,离心20min(8000r/min),取上清液作为样液。再用藤黄微球菌作为检定菌进行含量测定。结果显示,泰乐菌素在0.1~0.8μg/ml的浓度范围内具有良好线性关系(r=0.9982),禽蛋中3种添加浓度2.00、4.00、8.00μg/ml的平均回收率为86.6%(n=9),RSD分别为5.1%、4.3%、3.9%(n=3)。

The microbiologie determination method of tylosin residue in birds eggs

This is a method for the quantitative analysis of tylosin residue in birds eggs by microbiologic test.Sample preparation:homogenization sample 1 minutes (5 000 r/min),weigh 20 g in a centrifugal tube,add 5 ml distilled water,mix throughly,deposit 60 minutes,centrifuge 20 minutes(8 000 r/min),remove the upper clearlayer,assay the supernatant by Micrococcus luteus.The results show that Tylosin has a good linear (r=0.998 2) in range of 0.1 μg/ml to 0.8 μg/ml.The mean recovery is 86.6% (n=9) for additional concentration 2.00 μg/ml,4.00 μg/ml and 8.00 μg/ml.The respective RSD is 5.1%,4.3% and 3.9% (n=3).