| 弓形虫直接凝集试验改良法的研究 |
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| 引用本文： | 张述义,郑思民.弓形虫直接凝集试验改良法的研究[J].中国兽医寄生虫病,1994,2(2):11-13. |
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| 作者姓名： | 张述义 郑思民 |
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| 作者单位： | 上海市寄生虫病防治研究所 |
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| 摘 要： | 用弓形虫直接凝集试验改良法（ＭＤＡＴ）、染色试验（ＤＴ）和两种间接荧光抗体试验（ＩＦＡ）平行检测了国内外的７１份人血清，结果表明ＭＤＡＴ与其它试验符合率达９１．８～９６．０％；同时显示有较高的敏感性和特异性。用ＷＨＯ国际生物标准化实验室的标准血清定量测得ＭＤＡＴ的灵敏度为０．０９ＩＵ／ｍｌ，接近于改良前方法和ＤＴ的灵敏度，高于国外同类试剂盒的灵敏度。作者认为本ＭＤＡＴ具有在基层单位推广和应用的潜在价值。
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| 关 键 词： | 改良法 弓形虫 凝集试验 WHO 人血清 间接荧光抗体试验 符合率 推广 DT 染色试验 |
MODIFICATION AND STANDARDIZATION OF TOXOPLAMSMA DIRECT AGGLUTINATION TEST |
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| Zhang Shuyi,Zhang Simin.MODIFICATION AND STANDARDIZATION OF TOXOPLAMSMA DIRECT AGGLUTINATION TEST[J].Chinese Journal of Veterinary Parasitology,1994,2(2):11-13. |
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| Authors: | Zhang Shuyi Zhang Simin |
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| Institution: | Shanghai Institute of Parasitic Diseases Control and Researcb |
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| Abstract: | Toxoplasme direct agglutination test was modified and standardized that uses Formalin-fixed and Fuchsin basic stained tachyzoite antigen. The modified direct agglutination test (MDAT) was evaluated by comparing it with the dye test (DT) and two indirect fluorescent antibody (IFA) tests and by detecting its lowest level of antibodies. Results for 71 serum specimens indicated that good qualitative agreement exists among these tests (91.8 % to 96.0% ). The MDAT,standardized to the WHO reference, enables detection of antibodies doWn to a level of apptoalmately 0.09 IU/ml. which was similar tO 0.08 IU/ml for original DAT and 0.11 IU/ml for the DT described by Desmonts and Remington (1980). As the MDAT is economical and easy to Perform, it is recommended for mass screening and sero-epidemiological studies of Toxoplasma infection. |
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| Keywords: | Toxoplasma gondii direct agglutination test(DAT) dye test(DT) indirect fluorescent antibody (IFA) |
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