| 伊氏锥虫在家兔体内的抗原变异和NaTat1．1—1．9九个群体的建立 |
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| 引用本文： | 方元,叶万祥,聂海洋,王元伦.伊氏锥虫在家兔体内的抗原变异和NaTat1．1—1．9九个群体的建立[J].中国兽医寄生虫病,1994,2(2):5-10. |
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| 作者姓名： | 方元 叶万祥 聂海洋 王元伦 |
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| 作者单位： | 南京军区军事医学研究所 |
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| 摘 要： | 以伊氏锥虫YNB2克隆感染家兔，于52天内，以一定间隔，用经环磷酰胺处理的小鼠由兔血分离锥虫，并予以克隆，获得9个抗原性互不相同的克隆群体。用此9个克隆群体分别免疫家兔制备免疫血清，与同源和各异源克隆群体作交叉免疫溶解和间接免疫荧光试验。结果证明，9个克隆群体是单一可变抗原型(VAT)，制备的血清是单价VAT特异血清。按国际通用命名法，依分离顺序命名为NaTat1．1～1．9(Najing rypanozoon antigen type 1.1～1.9).
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| 关 键 词： | 克隆 体内 家兔 血分 VAT 环磷酰胺 免疫血清 伊氏锥虫 群体 抗原变异 |
ANTIGENIC VARIATION OF TRYPANOSOMA EVANSI IN RABBIT AND ESTABLISHMENT OF NaTat 1. 1-1. 9 VARIABLE ANTIGEN TYPE POPULATIONS |
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| Fang Yuan et al.ANTIGENIC VARIATION OF TRYPANOSOMA EVANSI IN RABBIT AND ESTABLISHMENT OF NaTat 1. 1-1. 9 VARIABLE ANTIGEN TYPE POPULATIONS[J].Chinese Journal of Veterinary Parasitology,1994,2(2):5-10. |
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| Authors: | Fang Yuan |
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| Institution: | The Military Medical Institute in Nanjing Military Area |
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| Abstract: | A cloned stock of Trypanosoma evanst was shown to undergo antigenic variation giving rise to nine antigenically different trypanosomes populations during the first 52 days of infection in a rabbit. Clones of the 9 trypanosomes populations were set up in mice treated with cyclophosphamide. Specific antisera against each of the 9 clones were produced in rabbits. These antisera were used for characterizaion and comparison of the 9 clones with each other by means of immunolysis and lmmunofluotescence tests. The results demonstrated that all the 9 clones were antigenically different mono-VAT populations and these antisera mono-VAT specific sera. The group of mono-VAT populations were designated as NaTat 1.1-1.9 (Nanjing Trypanozoon antigen type 1. 1-1 .9) according to Lumsden's nomenclature. |
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| Keywords: | Trypanosoma evansi clone antigenic variation variable antigen type surface variable antigen immunolysis lmmunofluoresecence NaTat 1 1-1 9 |
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