| 高效液相色谱法同时测定互叶白千层中的槲皮素和山奈酚 |
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| 引用本文： | 林霄,董晓敏,陈明生,黄艳,刘布鸣.高效液相色谱法同时测定互叶白千层中的槲皮素和山奈酚[J].林产化学与工业,2012,32(4):113-116. |
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| 作者姓名： | 林霄 董晓敏 陈明生 黄艳 刘布鸣 |
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| 作者单位： | 广西中药质量标准研究重点实验室,广西南宁530022;广西壮族自治区中医药研究院,广西南宁530022 |
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| 基金项目： | 广西自然科学基金资助项目 |
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| 摘 要： | 用高效液相色谱法同时测定广西5地互叶白千层中槲皮素和山奈酚含量。样品用盐酸-甲醇(体积比5∶100)混合溶液水浴加热回流提取,C18柱色谱分离,以甲醇-乙腈-0.4%磷酸(体积比21∶21∶58)水溶液为流动相,检测波长370 nm,柱温为室温,流速1 mL/min。测定了不同产地互叶白千层中的槲皮素和山奈酚质量分数,其中邕宁南晓的槲皮素和山奈酚的质量分数最高,分别为5.151和2.530 mg/g,邕宁的槲皮素和山奈酚的质量分数为最低,分别为2.386和1.658 mg/g。
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| 关 键 词： | 互叶白千层 槲皮素 山奈酚 高效液相色谱法 |
Simultanous Determination of Quercetin and Kaempferol in Melaleuca alternifolia (Maiden et Betehe) Chee 1 by HPLC |
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| LIN Xiao
,
DONG Xiao-min
,
CHEN Ming-sheng
,
HUANG Yan
,
LIU Bu-ming.Simultanous Determination of Quercetin and Kaempferol in Melaleuca alternifolia (Maiden et Betehe) Chee 1 by HPLC[J].Chemistry & Industry of Forest Products,2012,32(4):113-116. |
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| Authors: | LIN Xiao DONG Xiao-min CHEN Ming-sheng HUANG Yan LIU Bu-ming |
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| Institution: | 1,2(1.Guangxi Key Laboratory of Tradtitonal Chinese Medicine Quality Stardands,Nanning 530022,China; 2.Guangxi Institute of Traditional Medical and Pharmaceutical Sciences,Nanning 530022,China) |
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| Abstract: | A high-performance liquid chromatography(HPLC) method was developed for simultaneous determination of quercetin and kaempferol in Melaleuca alternifolia(Maiden et Betehe) Chee 1.The sample was extractd by HCl-MeOH solution(volume ratio 5 ∶ 100) in water bath,then separated by a C18 column with CH3OH-C2H3N-0.4% phosphoric acid solution(volume ratio 21 ∶ 21 ∶ 58) as the mobile phase solution under 370 nm wavelength.The HPLC experiment was performed at room temperature with flow rate 1.0 mL/min.Quercetin and kaempferol contents in M.alternifolia from different area were determined.The result showed that quercetin and kaempferol content in Nanxiao town of Yongning was the highest and that in Yongning was the lowest. |
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| Keywords: | Melaleuca alternifolia(Maiden et Betehe) Chee 1 quercetin kaempferol HPLC |
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