| 量子点标记荧光免疫法检测花生中黄曲霉毒素B1 |
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| 引用本文： | 李园园,,李培武,,﹡,张 奇,,张 文,,丁小霞,,张兆威,,.量子点标记荧光免疫法检测花生中黄曲霉毒素B1[J].中国油料作物学报,2012,34(4):438-442. |
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| 作者姓名： | 李园园 李培武 ﹡ 张 奇 张 文 丁小霞 张兆威 |
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| 作者单位： | 1.中国农业科学院油料作物研究所，湖北 武汉，430062；
2.农业部油料作物生物学与遗传育种重点实验室，武汉，430062；
3.农业部生物毒素检测重点实验室，湖北 武汉，430062；
4.农业部油料产品质量安全风险评估实验室（武汉），湖北 430062；5.农业部油料及制品质量监督检验测试中心，湖北 武汉，430062）
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| 基金项目： | 农业部948重点项目(2010-G1,2011-G5);国家自然科学基金(31171702,31101299);国家农业行业科技专项(201203094,201203037);国家科技支撑计划(2012BAB19B09,2011BAD02D02,2010BAD01B07);湖北省自然科学基金(2011CDB358) |
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| 摘 要： | 建立了基于量子点标记二抗的间接竞争荧光免疫吸附测定方法（indirect competitive fluorescence-linked immunosorbent assay，cFLISA），并研究检测花生中黄曲霉毒素B1可行性。采用谷胱甘肽为稳定剂，在水相中直接合成碲化镉（CdTe）量子点，并利用EDC与兔抗鼠二抗进行共价偶联，以黄曲霉毒素B1的单克隆抗体建立cFLISA方法。结果表明，该方法的灵敏度和最低检测限值分别为0.023ng/mL和0.001ng/mL，与传统的有机染料FITC-二抗法比较，灵敏度提高了30倍，花生样品加标0.1、0.05和0.025ng/g，回收率范围在88%~116%之间，变异系数均小于10%。建立的cFLISA方法可以较好的检测花生中黄曲霉毒素B1，并为其它真菌毒素的检测提供参考。
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| 关 键 词： | 碲化镉量子点 荧光免疫测定 黄曲霉毒素B1 花生 |
Fluoroimmunoassay using quantum dots label for detecting of aflatoxin B1 in peanuts |
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| LI Yuan-yuan,LI Pei-wu,ZHANG Qi,ZHANG Wen,DING Xiao-xia,ZHANG Zhao-wei.Fluoroimmunoassay using quantum dots label for detecting of aflatoxin B1 in peanuts[J].Chinese Journal of Oil Crop Sciences,2012,34(4):438-442. |
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| Authors: | LI Yuan-yuan LI Pei-wu ZHANG Qi ZHANG Wen DING Xiao-xia ZHANG Zhao-wei |
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| Institution: | 1,2,3,4(1.Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan 430062,China; 2.Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Wuhan 430062,China; 3.Key Laboratory of Detection for Mycotoxins,Ministry of Agriculture,Wuhan 430062,China; 4.Laboratory of Risk Assessment for Oilseeds Products(Wuhan),Ministry of Agriculture,Wuhan 430062,China; 5.Quality Inspection and Test Center for Oilseeds Products,Ministry of Agriculture,Wuhan 430062,China) |
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| Abstract: | System of indirect cFLISA(competitive fluorescence-linked immunosorbent assay) was established and evaluated for detecting of peanut aflatoxin B1(AFB1) using CdTe quantum dots as the fluorescence label coupled with secondary antibody.The glutathione modified water-soluble CdTe QDs was synthesized and covalent coupled with the secondary antibody by 1-ethlyl-3-(3-dimethylaminoproyl) carbodiimide hydrochloride(EDC).And the monoclonal antibody was used to against AFB1.The 50% inhibition value and the limit of detection(LOD) of the cFLISA were 0.023ng/mL and 0.001ng/mL respectively.Compared to organic dye FITC-secondary antibody,the sensitivity of this method was 30-fold higher than that of the traditional method.Recoveries of cFLISA from different spiked peanut samples were from 88% to 116% with a coefficient of variation less than 10%.Result indicated that cFLISA could be used for the detection of AFB1 in peanuts,and could also be a valuable reference to other mycotoxins application. |
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| Keywords: | CdTe Fluoroimmunoassay AFB1 Peanuts |
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