亚临界流体萃取油茶籽油的抗氧化活性研究

分析亚临界萃取油茶籽油、市售精炼油茶籽油和压榨毛油中多酚含量差异,对比不同工艺油茶籽油对1,1-二苯基-2-苦基肼自由基(DPPH.)清除、葵花籽油自氧化过程中过氧化值和硫代巴比妥酸值的影响。结果表明,亚临界油茶籽油、市售精炼油茶籽油和压榨毛油中多酚质量分数分别为6.26、1.70和8.33 mg/g;亚临界油茶籽油的DPPH.半数抑制质量浓度(IC50)为15.39 g/L(VE0.02 g/L),与压榨毛油相当,而明显低于市售精炼油茶籽油(21.66 g/L),表明亚临界油茶籽油和压榨毛油含有较多的酚类物质,同时亚临界油茶籽油抑制葵花籽油贮藏过程中过氧化值和硫代巴比妥酸值的能力强于压榨毛油和市售精炼油茶籽油。亚临界流体萃取油茶籽油有利于维持茶油产品的的抗氧化能力,在油茶籽油的加工过程中,亚临界萃取工艺在茶油产品生产上具有优越性。

Antioxidant Activity of Camellia Oil Extracted by the Sub-critical Fluid

In this paper,the polyphenol contents,DPPH-scavenging ability and effects of inhibition of the peroxide value and thiobarbituric acid value of sunflower oils during storage were analyzed for sub-critical camellia oil,commercial refined camellia oil and pressed crude camellia oil.It was shown from the results that the polyphenol contents of of sub-critical camellia oil,commercial refined camellia oil and pressed crude camellia oil were 6.26 mg/g,1.70 mg/g and 8.33 mg/g,respectively.The DPPH radical scavenging IC50 of sub-critical camellia oil was 15.39 g/L(equivalent to 0.02 g/L of VE),which was approximate to pressed crude camellia oil,but significantly lower than the commercially available refined camellia oil(21.66 g/L).There were much more polyphenols contained in both sub-critical camellia oil and pressed crude camellia oil.The sub-critical camellia oil had a better performance in inhibiting the peroxide value and thiobarbituric acid value of sunflower oils during storage than that of commercial refined camellia oil and pressed crude camellia oil.Sub-critical camellia oil had the capacity to keep the antioxidant activity of oil products.In the process of producing camellia oil,sub-critical fluid extraction was a superior technology.