| 根癌农杆菌农杆菌介导抗菌肽基因转化大豆的研究 |
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| 引用本文： | 李春风,张兴政,张洁莉,韩阳,宋书宏.根癌农杆菌农杆菌介导抗菌肽基因转化大豆的研究[J].中国油料作物学报,2012,34(4):348-352. |
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| 作者姓名： | 李春风 张兴政 张洁莉 韩阳 宋书宏 |
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| 作者单位： | 1.辽宁大学生命科学院，辽宁 沈阳， 110036；
2.辽宁省农业科学院作物研究所，辽宁 沈阳， 110161
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| 基金项目： | 辽宁省教育厅重点实验室项目(LS2010070) |
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| 摘 要： | 为利用抗菌肽基因培育转基因大豆抗病材料，采用根癌农杆菌介导法将抗菌肽基因和绿色荧光蛋白基融合因转入大豆胚尖外植体。结果表明，60mg/L的卡那霉素可以对转化植株进行有效的筛选；在共培养时添加200µmol/L乙酰丁香酮有利于抗菌肽基因的转化；在生根培养基中不添加卡那霉素更有利于转化苗的成活。目的基因特异的PCR检测和绿色荧光蛋白表达分析，发现抗菌肽基因已转入大豆，并得到表达。
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| 关 键 词： | 大豆 遗传转化 胚尖外植体 抗菌肽基因 根癌农杆菌介导 |
Transformation of anti-microbial peptides gene into soybean via Agrobacterium-mediation |
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| LI Chun-feng,ZHANG Xing-zheng,ZHANG Jie-li,HAN Yang,SONG Shu-hong.Transformation of anti-microbial peptides gene into soybean via Agrobacterium-mediation[J].Chinese Journal of Oil Crop Sciences,2012,34(4):348-352. |
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| Authors: | LI Chun-feng ZHANG Xing-zheng ZHANG Jie-li HAN Yang SONG Shu-hong |
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| Institution: | 1.School of Life Sciences,Liaoning University,Shenyang 110036,China; 2.Crops Research Institute,Liaoning Academy of Agricultural Sciences,Shenyang 110161,China) |
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| Abstract: | A anti-microbial peptide and green fluorescent protein(GFP) fusion gene was transformed into soybean embryos tips via Agrobacterium-mediation.Result showed 60mg/L kanamycin could effectively select the transformed plants,and 200μmol/L acetosyringone in coculture medium could improve gene transduction efficiency.No kanamycin was added in rooting medium for it was found harmful to root.PCR result and expression of green fluorescent protein(GFP) indicated that the target gene was transformed into soybean and correctly expressed. |
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| Keywords: | Soybean Transformation Embryos tip explant Anti-microbial peptide gene Agrobacterium-mediated |
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