用梯度聚丙烯酰胺凝胶垂直板型电泳法对雌、雄梭鱼血清蛋白的研究

实验采用梯度和单一浓度聚丙烯酰胺凝胶垂直板型电泳的方法，对梭鱼血清蛋白进行分析，其中梯度浓度(4．75％ 7％)凝胶分离梭鱼血清蛋白得30条区带，比单一浓度(4．75％、7％、10％)凝胶分离的效果好，区带清晰，带数明显增加。同时发现0号、2号、5号、8号和12号雌梭鱼血清蛋白电泳图型中发现有一明显深带，此深带在雄鱼的血清蛋白电泳图型中则没有发现。在数次的重复实验中，结果完全一致。作者认为此深带可能与雌性特异血浆蛋白有关。

INYESTIGATIUN OF GRADIENT PDLYACRYLAMIDE GEL SLAB-ELECTRaPHDRESIS ON SERUM PRDTEIN OF SEXUAL MULLETS (MUGIL SO-IUY BASYLEWSKY)

Whole sera of Mullet (Mugil so-iuy Bffsilewsky) analysed by electrophoresis acryamide discontinuous gradient gel (4 .75%+7%)showed 25-30 bands. The highest mobility fraction consists of albumin components and several preprotein components. The largest band is composed mainly of albumin components. Liporoteins with large iuolecular size axe localized at the negatiVo pole. A specific protein hand was observed in the sera of the female fish by discontinuous gradient polyacrylamide gel but was not found in the males. The specific female serum protein migrates as a protein of medium molecular site which is distinctly different from albumin arad. globulin. Thus discontinuous gradient polyacylamide gel electrophoresis affords a highly sensitive and more practical teehnique for analysis of proteins.