| 一种快速、有效的香蕉枯萎病病原菌FOC4的分子检测方法 |
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| 引用本文： | 叶建军,朱军,黄惠琴,方哲,鲍时翔. 一种快速、有效的香蕉枯萎病病原菌FOC4的分子检测方法[J]. 中国生物防治, 2008, 0(Z1) |
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| 作者姓名： | 叶建军 朱军 黄惠琴 方哲 鲍时翔 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所; |
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| 基金项目： | 中央级公益性科研院所基本科研业务费资助 |
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| 摘 要： | 以ITS测序与SCAR分子标记相结合的香蕉病原菌FOC4分子检测方法,利用通用引物ITS1和ITS4对病原菌SF001的rDNA中ITS序列进行PCR扩增,获得560bp左右的特异条带。经Blast分析比对,确定该菌为FOC。再利用FOC4的特异引物PCL/PDL对基因组上的特征序列扩增区域(SCAR)进行PCR扩增,获得677bp的特有序列,鉴定菌株SF001是尖孢镰刀菌古巴专化型4号生理小种。经过致病性测试,菌株SF001表现出典型4号生理小种的病理特征。
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| 关 键 词： | 香蕉枯萎病(尖孢镰刀菌古巴专化型) 分子检测方法 |
A Rapid and Effective Molecular Detection Method of the Pathogen FOC4 of Fusarium Wilt Disesase of Banana |
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| YE Jian-jun,ZHU Jun,HUANG Hui-qin,FANG Zhe,BAO Shi-xiang. A Rapid and Effective Molecular Detection Method of the Pathogen FOC4 of Fusarium Wilt Disesase of Banana[J]. Chinese Journal of Biological Control, 2008, 0(Z1) |
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| Authors: | YE Jian-jun ZHU Jun HUANG Hui-qin FANG Zhe BAO Shi-xiang |
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| Affiliation: | YE Jian-jun,ZHU Jun,HUANG Hui-qin,FANG Zhe,BAO Shi-xiang~* (Institute of Tropical Bioscience , Biotechnology,CATAS,Haikou 571101,China) |
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| Abstract: | The method combining morphology characteristics with pathogenic experiments was always used for detection of Fusarium oxysporum f.sp.cubense(FOC).Because it was time-consuming and the experiment result was affected by many factors,this method was not good for the fast prevention and control of banana wilt disease.The purpose of this study was to establish a molecular detection method of banana pathogens FOC4 by internal transcribed spacer(ITS) sequencing and a specific molecular markers technology of sequen... |
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| Keywords: | Fusarium oxysporum f.sp.cubense molecular detection method |
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