应用GOT同工酶进行柑桔分类的研究

对柑桔亚科11个属52个种类、品种的GOT同工酶进行了分析。结果表明,各属之间谱带差异显著,基因型各异,但金柑属GOT-2谱带与酒饼簕属相一致,表明有可能酒饼簕是进化至金柑属的先头属,而印度枳属有可能是枳的先头属,试验结果支持Scora关于枸橼、柚和宽皮柑桔为柑桔亚属三个基本种的观点,但宽皮柑桔的原始种应当是台湾山桔或类似的野生桔。根据GOT酶带和形态分析,我们认为来檬由大翼橙进化而来;粗柠檬是(木黎)檬和柠檬的杂种;北京柠檬与巴甘檬是来檬与柠檬的杂种;橙类由宽皮柑桔与柚杂交产生。我们将小林神果属蚝壳刺属及酒饼簕属GOT-1带中出现的迁移速度快于P带的2条新带分别命名为H和G带。

A STUDY ON THE TAXONOMY OF CITRUS WITH GOT ISOZYMES

Isozymes of glutamate oxaloacetate transaminase ( GOT ) were ana-lyzed by the electraphoresi for 52 species and cultivars of 11 genera of Aurantiae. The results indicated that the pattern and genotype showed a great range of variability among genera, but Fortunella and Atalantia had the same GOT-2 pattern,indicating that Atalantia might be the pio-neer genus of Foriunella,and Aegle the pioneer genus of triforiate oran-ge. The results of the present study supported the view-point of Scora that citron, pummelo and mandarin are three elementary species of Citrus. However, the initial species of mandarin should be Tachibana or similar wild species. According to GOT-2 bands and their shapes, we propose that lime evolves from papeda, rough lemon is a hybrid between limonia and lemon, Meyer lemon and bergamot are hybrids of lime and lemon,oran-ges come from hybridization of pummelo and mandarin. Two new bands which moved faster than P band in GOT-1 of Feroniella, Seuerinia and Atalantia were named G and H respectively.