厚壳贻贝早期幼虫低温保存的影响研究

探讨低温(4 ℃)条件下的厚壳贻贝早期幼虫保存可能性,同时调查了不同培育密度对低温保存的影响。在正常条件下,继续培育低温保存后的幼虫,并调查其存活率和生长的变化。 结果表明,在低温保存后,早期幼虫存活率较高,超过95%;厚壳贻贝早期幼虫的壳长和壳高出现显著性的增长。不同培育组间,培育密度对厚壳贻贝早期幼虫的存活和生长影响不同,表明密度是幼虫低温保存的一个重要因素。在正常培育条件下,低温保存后的幼虫,3周后其存活率明显低于对照组,但仍超过50%,且其生长速度明显高于对照组。因此,低温培育是保存厚壳贻贝早期幼虫的有效方法,可用于今后贝类幼虫生物学实验和人工育苗技术的改善研究。

Study on refrigeration of the early larvae of the mussel Mytilus coruscus

The possibility of refrigeration(4 ℃)of early larvae of the mussel Mytilus coruscus was examined in the present study.The effect of larval density on the refrigeration of M.coruscus was also investigated in three different culture batches.After refrigeration,larvae were transferred to 18 ℃,and survival rates and shell growth of larvae were investigated.The present results showed that the average survival rates of early larvae were higher than 95%,and both shell length and shell height showed a significant increase during the 1-month refrigeration period.Larval densities of different culture batches had a different effect on larval survival and shell growth during refrigeration,indicating that larval density may be an important factor for larval refrigeration.After larvae were transferred and cultured at 18 ℃ for three weeks,survival rates of refrigerated larvae decreased to 56%,which was lower than that in control larvae(75%).In contrast,the shell growth rate of refrigerated larvae was higher than that of control larvae.Thus,refrigeration can be used as an effective method of storing larvae of M.coruscus for use in field of mollusk larval biology and improvement of seed production techniques in aquaculture industry.