| 野生大豆与栽培大豆的同工酶分析 |
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| 引用本文： | 韩琳娜,周凤琴.野生大豆与栽培大豆的同工酶分析[J].大豆通报,2010(1):18-20. |
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| 作者姓名： | 韩琳娜 周凤琴 |
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| 作者单位： | 山东中医药大学药学院; |
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| 基金项目： | 国家海洋局黄河三角洲湿地专项(908-01-ST12,908-02-05-04) |
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| 摘 要： | 本文采用聚丙烯酰胺凝胶电泳法,分析了黄河三角洲野生大豆与栽培大豆的过氧化物酶(POD)和酯酶(EST)同工酶谱。结果表明,二者过氧化物同工酶谱在数目、分布上存在比较明显的差异,酯酶同工酶也存在区别,过氧化物酶同工酶谱可以作为野生大豆和栽培大豆进化和类型鉴定的参考依据。POD相似系数为0.667,EST相似系数为0.714,说明二者亲缘关系较近。
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| 关 键 词： | 野生大豆 栽培大豆 过氧化物酶 酯酶 PAGE |
Isoenzyme analysis of Glycine soja and Glycine max |
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| Han Linna,Zhou Fengqin.Isoenzyme analysis of Glycine soja and Glycine max[J].Soybean Bulletin,2010(1):18-20. |
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| Authors: | Han Linna Zhou Fengqin |
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| Abstract: | By using the method of polyaerulamide gel eleetrophoresis,the esterase(EST) and peroxidase(POD) isozyme of Glycine soja and Glycine max were analyzed in the paper. The result showed that the difference of cultivars was not distinct in zymogram. The characteristics and distributions of EST and POD zymograms showed the differences between cultivated soybeans and wild soybeans. Based on the similarity coefficient of POD was 0.667,and the EST's was 0.714; therefore,the relationship of them was close to each oth... |
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| Keywords: | Wild soybean Cultivated soybean POD EST PAGE |
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