Identification of botanical origin of starches by SDS-PAGE analysis of starch granule-associated proteins |
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Authors: | Jae-Wook Yoon Jae-Yeon Jung Hyun-Jung Chung Mi-Ryung Kim Chan-Wha Kim Seung-Taik Lim |
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Affiliation: | 1. Graduate School of Life Sciences and Biotechnology, Korea University, 5-1 Anam-dong, Sungbuk-ku, Seoul 136-701, South Korea;2. Department of Bio-Food Materials, Silla University, San 1-1 Gwaebop-dong, Sasang-gu, Busan 617-736, South Korea |
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Abstract: | Starch granule-associated proteins (SGAPs) were extracted from various starches and analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with silver staining. The SDS-PAGE results showed that granule-bound starch synthase (GBSS) of approximately 60 kDa was found in most starches, except waxy-type starches. Starches exhibited the presence of proteins specific to the botanical origin, including those of 22 kDa for maize; 160 and 98 kDa for potato; 140, 115, 90, and 80 kDa for wheat. These proteins could be detected from noodles prepared with the corresponding starches. The detection of the specific proteins by SDS-PAGE may be used to identify the origin of starch incorporated in various foods and industrial products. |
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Keywords: | Starch granule-associated protein (SGAP) SDS-PAGE Starch noodle Botanical origin |
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