| 黑木耳茵丝原生质体的制备再生及单核鉴定研究 |
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| 引用本文： | 许修宏,孟琦,刘华晶,李亮. 黑木耳茵丝原生质体的制备再生及单核鉴定研究[J]. 东北农业大学学报, 2011, 42(8): 96-100 |
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| 作者姓名： | 许修宏 孟琦 刘华晶 李亮 |
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| 作者单位： | 1. 东北农业大学资源与环境学院,哈尔滨,150030
2. 黑龙江省农业科学院大庆分院,黑龙江大庆,161090 |
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| 基金项目： | 科技部林业公益性行业科研专项 |
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| 摘 要： | 探讨了黑木耳原生质体的制备、再生及单核化的问题.结果表明,培养了8d的黑木耳茵丝体用硫酸铗做稳渗剂,2.0%的溶壁酶酶解,31℃下水浴4h所产生的原生质体数量最多,可以达到11.5×107个·mL-1,0.5 mo1·mL-1硫酸镁渗透压稳定剂,RM5再生培养基条件下再生率最高,最高可达1.63%.原生质体再生的茵丝接...
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| 关 键 词： | 黑木耳 原生质体 再生 单核 |
Study on protoplast isoulation and regeneration and monokaryotization of Auricularia auricula |
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| XU Xiuhong,MENG Qi,LIU Huajing,LI liang. Study on protoplast isoulation and regeneration and monokaryotization of Auricularia auricula[J]. Journal of Northeast Agricultural University, 2011, 42(8): 96-100 |
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| Authors: | XU Xiuhong MENG Qi LIU Huajing LI liang |
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| Affiliation: | 1.College of Resources and Environmental Sciences,Northeast Agricultural University,Harbin 150030,China;2.Heilongjiang Academy of Agricultural Sciences,Daqing Heilongjiang 161090,China) |
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| Abstract: | The highest yield of protoplast could be obtained by using 2% lywallzyme made by 0.5 mol·L-1 MgSO4·7H2O,digesting for 4 h at the condition of 31 ℃,the production of protoplast reached 11.5×106 number·mL-1,0.5 mol·L-1 magnesium sulfate osmotic stabilizer,RM5 regeneration medium under the conditions of the highest recycling rate up to 1.63%.Regeneration of mycelial protoplasts inoculated Petri dishes on PDA medium comprehensive,25 ℃ static culture.After the plate to be covered with mycelium,cut with scissors the size of the square side length 1 cm transparent tape,with its sticky plate and the mycelium was observed under the microscope. |
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| Keywords: | Auricularia auricula protoplast regeneration monokaryotization |
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