| 基于线粒体COI基因部分序列分析4种松毛虫的亲缘关系 |
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| 引用本文： | 贾玉迪,孔祥波,张真,王鸿斌,王凤云,张生.基于线粒体COI基因部分序列分析4种松毛虫的亲缘关系[J].东北林业大学学报,2011,39(11):67-70,78. |
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| 作者姓名： | 贾玉迪 孔祥波 张真 王鸿斌 王凤云 张生 |
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| 作者单位： | 1. 国家林业局森林保护学重点实验室(中国林业科学研究院森林生态环境与保护研究所),北京,100091
2. 河北省平泉县林业局 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金，国家自然科学基金 |
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| 摘 要： | 为了探讨4种松毛虫的亲缘关系,对马尾松毛虫、油松毛虫、赤松毛虫和落叶松毛虫的线粒体COI基因序列进行了扩增和测序.在获得的636 bp的COI序列中碱基的平均频率为:A=34.75％,T/U =34.75％,C=15.25％,G=15.25％.马尾松毛虫遗传多样性最高(核酸多样度为3.93％),落叶松毛虫最低(0.62...
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| 关 键 词： | 马尾松毛虫 油松毛虫 赤松毛虫 落叶松毛虫 C0I 亲缘关系 |
Taxonomic Position of Four Species of Pine Caterpillar Moths Based on Sequences Analysis of COI Gene |
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| Jia Yudi,Kong Xiangbo,Zhang Zhen,Wang Hongbin,Wang Fengyun,Zhang Sheng.Taxonomic Position of Four Species of Pine Caterpillar Moths Based on Sequences Analysis of COI Gene[J].Journal of Northeast Forestry University,2011,39(11):67-70,78. |
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| Authors: | Jia Yudi Kong Xiangbo Zhang Zhen Wang Hongbin Wang Fengyun Zhang Sheng |
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| Institution: | (Pingquan Forestry Bureau of Hebei Province) |
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| Abstract: | Genetic differentiation among four species of Dendrolimus punctatus,D.tabulaeformis,D.spectabilis,and D.superans was analyzed based on COI gene sequences in order to clarify the taxonomic status of the four pine moths.The average base frequencies of 636bp COI gene sequences were obtained as A 34.75%,T/U 34.75%,C 15.25%,and G 15.25%.D.punctatus has the highest genetic diversity(3.93%),while the genetic diversity of D.superans is the lowest(0.629%).The genetic distance among the four spices is in the order of D.superans × D.spectabilis(0.083),D.superans × D.punctatus(0.068),D.superans × D.tabulaeformis(0.066),D.spectabilis × D.tabulaeformis(0.057),D.punctatus × D.spectabilis(0.054),and D.punctatus × D.tabulaeformis(0.012).The COI sequence can be used to identify the genetic relationship between the four species of pine months and is a suitable marker in Dendrolimus molecular taxonomy. |
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| Keywords: | Dendrolimus punctatus D tabulaeformis D spectabilis D superans COI Genetic relationship |
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