| 对二甲氨基苯甲醛显色分光光度法检测水溶液中常微量尿素 |
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| 引用本文： | 苗晓杰,蒋恩臣,王佳,杜衍红. 对二甲氨基苯甲醛显色分光光度法检测水溶液中常微量尿素[J]. 东北农业大学学报, 2011, 42(8): 87-92 |
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| 作者姓名： | 苗晓杰 蒋恩臣 王佳 杜衍红 |
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| 作者单位： | 华南农业大学工程学院,广州,510640 |
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| 基金项目： | 广东省科技厅社会发展项目 |
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| 摘 要： | 对二甲氨基苯甲醛能够在酸性条件下和尿素反应生成柠檬黄色物质,可依此用比色法测定尿素含量.文章通过条件实验和精密度准确度实验,建立了利用对二甲氨基笨甲醛显色分光光度法检测水溶液中常微量尿素的标准曲线和具体测试方法.选择测试波长为422 nm,对二甲氨基苯甲醛(PDAB)显色剂用量10 mL,硫酸溶液用量4 mL,显色时间...
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| 关 键 词： | 对二甲氨基苯甲醛 分光光度法 尿素 测定 |
Using spectrophotometry with para-dimethyl-amino-benzaldehyde as chromogenic agent to determine macro and micro urea in aqueous solution |
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| MIAO Xiaojie,JIANG Enchen,WANG Jia,DU Yanhong. Using spectrophotometry with para-dimethyl-amino-benzaldehyde as chromogenic agent to determine macro and micro urea in aqueous solution[J]. Journal of Northeast Agricultural University, 2011, 42(8): 87-92 |
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| Authors: | MIAO Xiaojie JIANG Enchen WANG Jia DU Yanhong |
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| Affiliation: | (Engineering College,South China Agricultural University,Guangzhou 510640,China) |
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| Abstract: | Lemon-yellow material will be produced when para-dimethyl-amino-benzaldehyde(PDAB) reacts with urea under acidic condition,which can be used to determine the macro and micro urea concentration in aqueous solution with colorimetry.Standard curve and specific testing method regarding using spectrophotometry to determine urea concentration with PDAB as chromogenic agent were established through condition experiment and precision and accuracy experiment in the paper.422 nm was selected as testing wavelength,10 mL as the addition dosage of PDAB solution,4 mL as sulfuric acid addition dosage and 10 min as reaction time.Under the conditions,the standard curve obtained embodied highly significant linear relationship ranging 0-1 000 μg·mL-1 and the detection limit was 0.5 μg·mL-1.The method has high accuracy and precision as well as good stability and reliability. |
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| Keywords: | para-dimethyl-amino-benzaldehyde spectrophotometry urea determination |
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