| 美洲南瓜裸仁基因的AFLP和SCAR分子标记分析 |
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| 引用本文： | 李智媛,屈淑平,崔崇士.美洲南瓜裸仁基因的AFLP和SCAR分子标记分析[J].东北农业大学学报,2011,42(7):67-71. |
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| 作者姓名： | 李智媛 屈淑平 崔崇士 |
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| 作者单位： | 东北农业大学园艺学院,哈尔滨,150030 |
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| 基金项目： | 东北农业大学科技创新团队项目 |
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| 摘 要： | 以美洲南瓜有壳品系“金辉二号-2”和无壳品系“0516-2”进行杂交,获得193个F2单株作为作图群体,利用AFLP分子标记技术和集群分析法(BSA)对与美洲南瓜裸仁基因连锁的分子标记进行研究.结果表明,找到了与裸仁基因n连锁的4个AFLP标记:E19M60-C、E22M48-B、E13M60-A和E25M51-D,连...
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| 关 键 词： | 美洲南瓜 裸仁基因n AFLP SCAR |
Development of AFLP and SCAR markers linked to hull-less n gene in pumpkin(Cucurbita pepo L.) |
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| LI Zhiyuan,QU Shuping,CUI Chongshi.Development of AFLP and SCAR markers linked to hull-less n gene in pumpkin(Cucurbita pepo L.)[J].Journal of Northeast Agricultural University,2011,42(7):67-71. |
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| Authors: | LI Zhiyuan QU Shuping CUI Chongshi |
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| Institution: | (College of Horticulture,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | A population of pumpkin F2 generation including 193 individual plants was derived from the crossing between hull line Jinhui2-2 and hull less line 0516-2 in this study.AFLP and bulked segregant analyses(BSA)were used to identify molecular markers linked to hull-less traits in F2 population.Four AFLP markers were developed.The genetic distance among four AFLP markers E19M60-C,E22M48-B,E13M60-A,E25M51-D and hull-less seed gene were 8.9,14.1,17.5 and 18.8 cM,respectively.And E19M60-C was also successfully converted into a SCAR marker. |
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| Keywords: | Cucurbita pepo hull-less n gene AFLP SCAR |
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