| 谷胱甘肽对铜孵育的肉鸡肝细胞线粒体的保护作用 |
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| 引用本文： | 苏荣胜,闫文龙,潘家强,贾雪霞,李海琴,许冬蕾,唐兆新.谷胱甘肽对铜孵育的肉鸡肝细胞线粒体的保护作用[J].兽医大学学报,2012(4):602-605. |
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| 作者姓名： | 苏荣胜 闫文龙 潘家强 贾雪霞 李海琴 许冬蕾 唐兆新 |
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| 作者单位： | [1]华南农业大学兽医学院,广东广州510642 [2]韶关学院英东生物工程学院,广东韶关512005 |
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| 基金项目： | 国家自然科学基金资助项目（30871900）; 广东省自然科学基金资助项目（8451200501000533） |
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| 摘 要： | 向即时分离的肉鸡肝细胞线粒体培养液内加入终浓度分别为10、30、50μmol/L的Cu2＋和200μmol/L GSH＋50μmol/L Cu2＋,孵育20min后,观察培养液中不同铜浓度对线粒体H2O2生成速率和呼吸链复合物活性的影响。结果表明,30μmol/L和50μmol/L的Cu2＋能引起线粒体H2O2生成速率和呼吸链复合物活性出现明显加快和降低（P〈0.05或P〈0.01）,10μmol/L Cu2＋组没有出现明显变化;谷胱甘肽可显著抑制50μmol/L Cu2＋引起的变化（P〈0.01）,对线粒体具有保护作用。
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| 关 键 词： | 铜 线粒体 过氧化氢 线粒体复合物 谷胱甘肽 |
Protective effect of glutathione on copper exposure to liver mitochondria of broilers |
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| Authors: | SU Rong-sheng YAN Wen-long PAN Jia-qiang JIA Xue-xia LI Hai-qin XU Dong-lei TANG Zhao-xin |
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| Institution: | 1.College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;2.Yingdong Bioengineering College,Shaoguan University,Shaoguan,Guangdong 512005,China) |
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| Abstract: | Hydrogen peroxide（H2O2） generation and activities of respiratory chain complexes of mitochondria were observed after incubating the mitochondria which was freshly isolated from broiler hepatocyte with 10,30,50 μmol/L Cu2＋ and 200 μmol/L glutathione（GSH）＋50 μmol/L Cu2＋for 20 min,respectively.The values of H2O2 generation and activities of respiratory chain complexes advanced and decreased significantly correspondingly（P0.05 or P〈0.01） after Cu2＋ exposure at 30 and 50 μmol/L,and no obvious difference at 10 μmol/L Cu2＋.GSH could obviously inhibit changes of H2O2 generation and activities of respiratory chain complexes after Cu2＋ exposure at 50 μmol/L（P〈0.01） and protect mitochondria. |
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| Keywords: | copper mitochondria hydrogen peroxide mitochondrial complex glutathione |
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