猪瘟免疫猪接种PRRSV Nsp2Δ1882-2241弱毒疫苗后免疫应答及T细胞表型变化特征

选择17头28日龄的CSFV和PRRSV抗体均为阴性的仔猪，于试验的第1天和第14天分别对其进行猪瘟耐热保护剂活疫苗（兔源）和高致病性猪繁殖与呼吸综合征Nsp2A1882—2241弱毒疫苗免疫。在免疫后的第28、42天采集外周血液，分析特异性抗体表达量和外周血T淋巴细胞表型的变化，评估猪瘟免疫对猪繁殖与呼吸综合征免疫的影响。结果显示，在CSF免疫后第28、42天，CSFV高抗组中的CD4^＋、CD4^＋／CD8^＋、CD4^＋CD8^＋和CD4-CD8数目均比CSFV低抗组高，CD3^＋和CD8^＋细胞数量比CSFV低抗组低；PRRS高抗组中，CD4CD8-细胞含量高于PRRS低抗组；在CSF免疫后第28天，CSFV抗体产生较高（阳性比率为73．33％），PRRSV抗体产生较低（阳性比率仅为6．67％）。在CSF免疫后的第42天，CSF高抗组中PRRSV抗体阳性比率较CSF低抗组高8．33％。结果表明，CSFV特异性抗体产生高时能增加PRRSV特异性细胞免疫应答，增加CD4^＋细胞、CD4^＋CD8^＋细胞数量，提高机体免疫水平。CD3＋和CD4CD8-细胞应答作用值得重视。

Immune response and the changes of lymphocyte subpopulations in swine inoculated by porcine reproductive and respiratory syndrome Nsp2A1882-2241 attenuated vaccine after CSF vaccination

Seventeen 28-day-old pigs were selected from CSF-free, PRRS-free farm and then were vaccinated the swine fever thermo-stable vaccine,live （rabbit origin） and highly pathogenic porcine reproductive and respiratory syndrome vaccine,live （strain TJM-F92） according on day 1 and 14. On day 28 and 42 post vaccination,we collected the pe- ripheral blood for analyzing the amount of the special antibody and the changes of the lymphocyte subpopulations to assess the infection of the CSF immune to the PRRS immune. The result indicated that on day 28 and 42 post CSF vaccination,the numbers of CD4＋, CD4＋/CD8＋, CD4＋ CD8＋ and CD4-CDS- in high-CSFV antibody group were bigger than in low-CSFV antibody group,but the numbers of CD3＋ and CD8＋ were less than in low-CSFV antibody group. In high-PRRSV antibody group,the number of CD4-CD8- is bigger than in low-PRRSV antibody group. On day 28 post CSF vaccination,the CSFV antibody was higher （the positive ratio was 73.3%）, but PRRSV antibody was lower （the positive ratio was only 6.67% ）. On day 42 post CSF vaccination,in high-CSFV antibody group,the positive ratio of PRRSV antibody was 8.33% larger than in the low-CSF antibody group. This indicated that the large number of CSF special antibody can increase the amount of PRRSV special antibody, CD4＋ , CD4＋ CD8＋ and CD4- CD8- and enhance the level of immune. The immune repones of CD3＋ and CD4- CD8- cells is worth noticing.