HABP4基因在大白猪和莱芜猪杂交一代中表达量差异研究

采用实时荧光定量PCR技术检测透明质酸接合蛋白（HABP4）基因在大白猪和莱芜猪2种杂交子代背最长肌组织中的表达水平差异情况。试验结果表明,HABP4基因在所检测的背最长肌组织中均有表达。且在2种杂交一代背最长肌组织中的表达水平有所不同。HABP4基因的表达量在正交一代的背最长肌中显著高于反交一代。本试验为猪屠宰性状的改良提供了分子遗传学依据,并为深入研究不同品种猪体内HABP4基因的生物学作用及作用机制奠定了基础。

Differential expression analysis of HABP4 gene in the hybrid progeny of Large White and Laiwu pigs

HABP4 participates in a variety of important physiological and biochemical processes in mammalian, but only few studies have involved its exact physiological function and mechanism of action. In this study, using real-time quantitative PCR technology detected HABP4 gene expression levels in the muscle tissue of hybrid progeny of Large White and Laiwu pigs. The results showed that HABP4 gene was expressed in all longissimus muscle tissue detec- ted. And the expression level is different of longissimus muscle tissue in the two hybrid generation. HABP4 gene ex- pression in the longissimus muscle of the quadrature generation was significantly higher than anti-cross generation. The test provides the evidence of the molecular genetics basis to improve swine carcass traits, and laid the foundation of later in-depth study on the biological role and mechanism of the HABP4 gene in different pig breeds.