| 新疆褐牛核心群母牛蜘蛛腿综合征的遗传检测 |
| |
| 引用本文： | 席艾力·依明,王雅春,黄锡霞,田可川,田月珍,郭俊清,谭世新,付雪峰,热伊莱·伊卜拉伊木.新疆褐牛核心群母牛蜘蛛腿综合征的遗传检测[J].兽医大学学报,2012(9):1295-1298. |
| |
| 作者姓名： | 席艾力·依明 王雅春 黄锡霞 田可川 田月珍 郭俊清 谭世新 付雪峰 热伊莱·伊卜拉伊木 |
| |
| 作者单位： | [1]新疆农业大学动物科学学院,新疆乌鲁木齐830052 [2]中国农业大学动物科技学院,北京100193 [3]新疆畜牧科学院畜牧研究所,新疆乌鲁木齐830000 [4]新疆乌鲁木齐种牛场,新疆乌鲁木齐830049 [5]天山畜牧生物工程有限公司,新疆昌吉831109 |
| |
| 基金项目： | 国家“十二五”科技规划资助项目（2011BAD28B02） |
| |
| 摘 要： | 以173头新疆褐牛核心群母牛血样为基础,采用荧光引物PCR产物毛细管电泳法结合PCR产物直接测序法检测蜘蛛腿综合征隐性基因。结果显示,荧光引物PCR产物毛细管电泳法检测的173份血样DNA片段均在246～250bp出现单一信号峰。其中,21份PCR产物直接测序未出现牛蜘蛛腿综合征SUOX基因致病突变G碱基插入导致的叠峰现象,序列比对显示测序检测未发现突变的杂合子个体,22种方法检测结果一致。结果表明,2种检测方法所检测的新疆褐牛核心群母牛个体均为纯合基因型,没有发现牛蜘蛛腿综合征SUOX致病等位基因的存在或携带该致病基因的个体,说明该研究群体不存在传播牛蜘蛛腿综合征的风险。
|
| 关 键 词： | 新疆褐牛 蜘蛛腿综合征 毛细管电泳 测序 筛查 |
The Genetic detection of arachnomelia syndrome in the breeding group of Xinjiang brown cattle |
| |
| Institution: | YIMING XIaili, WANG Ya-chun , HUANG Xi-xia , TIAN Ke-chuan, TIAN Yue-zhen, GUO Jun- qing , TAN Shi-xin, FU Xue-feng , YIBULAYIMU Re yilai (1. College of Animal Science and Techonology , Xinjiang Agriculture University, Urumqi 830052, China;2. College of Animal Sci- ence and Technology, China Agriculture University, Beijing 100193, China; 3. Xinjiang Academy of Animal Scienc ,Urumqi 830000,China14. Breeding Cow Farm of Urumqi,Urumqi 8300499 China ; 5. Tianshan Animal Husbandry Bio-engineering Co. Ltd , Changj i , X inj iang 831109, China) |
| |
| Abstract: | 173 blood samples from core group of Xinjiang brown cows were used in this study. Using capillary elec- trophoresis combined with direct sequencing of PCR products tests arachnomelia syndrome mutation. All of the 173 samples tested by capillary electrophoresis showed a single signal peak among the 246-250 bp. 21 samples were fur- ther sequenced and no overlapping peaks caused by the G base insertion in SUOX was found. No heterozygous indi- viduals were detected by sequence alignment. The results of the two methods were consistent. The result showed the core group of Xinjiang brown cows is homozygous genotype and no SUOX allele exists,indicating that there is no risk of transmission of AS in this bovine group. |
| |
| Keywords: | Xinjiang Brown cattle arachnomelia syndrome capillary electrophoresis sequencing detection |
| 本文献已被 维普 等数据库收录！ |
|
