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饲料中鸡源性成分PCR-DHPLC检测方法的建立
引用本文:王金玲,贾金生,李俊环,张莹,王芳,高铁军. 饲料中鸡源性成分PCR-DHPLC检测方法的建立[J]. 兽医大学学报, 2012, 0(3): 493-496
作者姓名:王金玲  贾金生  李俊环  张莹  王芳  高铁军
作者单位:[1]沈阳出入境检验检疫局,辽宁沈阳110016 [2]锦州市义县动物卫生监督所,辽宁锦州121100
基金项目:沈阳市大型科学仪器设备共享专项资助项目(2007共享012)
摘    要:以鸡组织为材料,提取鸡组织的线粒体DNA,以鸡12SrRNA基因为靶基因设计引物,PCR反应扩增片段230bp;用13种畜禽和水产动物线粒体DNA验证该PCR引物的特异性,结果只有鸡线粒体DNA为阳性;PCR-DHPLC检测灵敏度在DNA水平上达到了2.04mg/L;在随机采集的22份饲料样品中,检出8份鸡源性成分样品。结果表明,本研究建立的PCR-DHPLC方法可特异、灵敏地对饲料中的鸡源性成分进行快速准确检测。

关 键 词:饲料  鸡源性成分  PCR-DHPLC

Establishment of PCR-DHPLC assay for detection of chicken tissue in feedstuff
WANG Jin-ling,JIA Jin-sheng,LI Jun-huan,ZHANG Ying,WANG Fang,GAO Tie-jun. Establishment of PCR-DHPLC assay for detection of chicken tissue in feedstuff[J]. , 2012, 0(3): 493-496
Authors:WANG Jin-ling  JIA Jin-sheng  LI Jun-huan  ZHANG Ying  WANG Fang  GAO Tie-jun
Affiliation:1.Shenyang Entry-Exit Inspection and Quarantine Bureau of P.R.C,Shenyang 110016,China;2.The Animal Health Supervision Yi Town in Jinzhou,Jinzhou,Liaoning 121100,China)
Abstract:By using chicken tissue as test material,selecting the mitochondrial DNA(mtDNA) 12S rRNA gene as marker gene,we designed capture probes,amplified a fragment of 230 bp.No cross-reaction was detected with other 13 animal mitochondrial DNA.The PCR-DHPLC had a detection limit of 2.04 mg/L genomic DNA for chicken tissue.22 feedstuff samples detected 8 chicken tissue positive.These results indicated that the PCR-DHPLC assay could be used for specific rapid detection of chicken tissue in feedstuff.
Keywords:feedstuff  chicken tissue  PCR-DHPLC
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