Seasonal sensitivity of stem water potential to vapour pressure deficit in grapevine

Boundary lines of stem water potential (Ψ_stem) responses to vapour pressure deficit (VPD) have been reported for several species and are generally referred to as VPD reference lines (RL). In order to study the response of Ψ_stem to VPD, RLs were determined in plants that received full (Control) and deficit (SSDI) irrigation during three consecutive years. The Control plants received irrigation equivalent to full crop water evapotranspiration minus effective rainfall and the SSDI plants were irrigated at 50% of the Control level. Ψ_stem values for the Control treatment during crop development tended to decrease, and those corresponding to SSDI plants were always lower than those for Control plants. Considering the 3-year data set, no relationship was found between Ψ_stem and VPD. However, there was a differential seasonal response between Ψ_stem and VPD, which depended on the stage of fruit development. A separate phenological analysis enabled the detection of RL for stage II (from fruit-set to veraison) and for stage III (post-veraison), whereas during stage I (from bud-break to fruit set) RL was not apparent. RL slopes increased as the season progressed and were significantly correlated to average values of Ψ_stem. The seasonal decrease in midday Ψ_stem for Control plants was interpreted as being a result of a progressive increase in canopy size and water consumption, which led to increased water depletion before each afternoon’s daily irrigation event. The apparent lack of RL during stage I was related to lower levels of water demand and high Ψ_stem.