| 靶向PRRSV核衣壳蛋白N基因的siRNA表达载体的构建及鉴定 |
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| 引用本文： | 曹素芳,李明,王岩,朱赞梅,刘长斗,唐桂芬,肖松云.靶向PRRSV核衣壳蛋白N基因的siRNA表达载体的构建及鉴定[J].农业科学与技术,2009,10(4):171-174. |
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| 作者姓名： | 曹素芳 李明 王岩 朱赞梅 刘长斗 唐桂芬 肖松云 |
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| 作者单位： | 曹素芳,李明,王岩,唐桂芬,肖松云(郑州牧业工程高等专科学校,河南郑州,450011);朱赞梅(河南大明药业有限公司,河南郑州,450027);刘长斗(郑州先锋动物药业有限公司,河南郑州,450032) |
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| 基金项目： | Based on Cutting-edge technology and research Project of Henan Province，The focus of Scientific and Technological Project of Henan Province，Colleges and Universities of Henan Province in Support of Technological Innovation Plan.河南省基础与前沿技术研究计划项目，河南省重点科技攻关，河南省高校科技创新人才支持计划 |
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| 摘 要： | 1材料与方法
1．1材料限制性核酸内切酶HindIII、BamHI、PvuII、SspI、质粒抽提试剂盒、EcoT14 DNAMarker购自TakaRa公司。T4DNA连接酶购于promega公司。大肠杆菌DH5α菌株由该实验室保存。siRNA表达载体pSilencer 4．1-CMV Nero质粒购自Ambion公司。
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| 关 键 词： | siRNA 表达载体 核衣壳蛋白 PRRSV N基因 限制性核酸内切酶 T4DNA连接酶 omega公司 |
Construction and Identification of siRNA Expression Vector Targeting Nucleocapsid Protein N gene of PRRSV |
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| CAO Su-fang,LIMing,WANG Yan,ZHU Zan-mei,LIU Chang-dou,TANG Gui-fen,XIAO Song-yun.Construction and Identification of siRNA Expression Vector Targeting Nucleocapsid Protein N gene of PRRSV[J].Agricultural Science ＆ Technology,2009,10(4):171-174. |
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| Authors: | CAO Su-fang LIMing WANG Yan ZHU Zan-mei LIU Chang-dou TANG Gui-fen XIAO Song-yun |
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| Institution: | 1. Zhengzhou College of Animal Husbandry Engineering, Zhengzhou 450011 ; 2. Henan Darning Animal Pharmaceutical Co., Ltd, Zhengzhou 450027; 3. Zhengzhou Xianfeng Animal Pharmaceutical Co,, Ltd, Zheng zhou 450032) |
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| Abstract: | Objective] The aim of this study was to investigate the construction and identification of siRNA expression vector targeting nucleocapsid protein N gone of PRRSV. Method] Three siRNA oligonucleotides targeting nucleocapsid protein N gone sequence of PRRSV were designed or synthesized, and then inserted into CMV promoter downstream to clone into pSilencer 4,1 -CMV eukaryotic expression vector. The recombinant expression vector was identified by enzyme digestion and DNA sequencing. Result] The results showed that the siRNA interference recombinant plasmid vector pSilencer-N targeting nucleocapsid protein gone expression had been successfully constructed. Conclusion] This study lays a foundation for studies on the controlling PRRSV by RNA interference technique . |
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| Keywords: | PRRSV Nucleocapsid protein N siRNA Expression vector |
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