| 甲基对硫磷降解菌DLL-1的诱变育种 |
| |
| 引用本文： | 刘智,李顺鹏.甲基对硫磷降解菌DLL-1的诱变育种[J].土壤学报,2003,40(2):293-300. |
| |
| 作者姓名： | 刘智 李顺鹏 |
| |
| 作者单位： | 南京农业大学资源与环境学院微生物学系,南京,210095 |
| |
| 基金项目： | 农业部“九五”重点项目 (编号 :96环 0 2 -0 2 )资助 |
| |
| 摘 要： | 采用紫外线和氯化锂诱变 ,从甲基对硫磷降解菌假单胞菌 (Pseudomonassp.)DLL -1中获取高效突变株DLL -E1 ,DLL -E2 ,DLL -E3 ,DLL -E4。并对其降解甲基对硫磷性能、降解对硝基苯酚性能、底物广谱性及胞外酶及粗酶液活性等生物学功能进行了研究。与出发菌株相比 ,上述性能均有所提高。选择DLL -E4作为进一步研究的对象 ,详细研究了其对甲基对硫磷及对硝基苯酚的耐受和降解情况。高效菌株的选育为农药残留降解的酶学研究打下了基础。
|
| 关 键 词： | 甲基对硫磷 假单胞菌 突变株 生物学功能 |
| 收稿时间： | 2001/2/15 0:00:00 |
| 修稿时间： | 2001年2月15日 |
MUTATION BREEDING OF METHYL PARATHION DEGRADING STRAIN DLL-1(PSEUDOMONAS sp.) |
| |
| Liu Zhi and Li Shun-peng.MUTATION BREEDING OF METHYL PARATHION DEGRADING STRAIN DLL-1(PSEUDOMONAS sp.)[J].Acta Pedologica Sinica,2003,40(2):293-300. |
| |
| Authors: | Liu Zhi and Li Shun-peng |
| |
| Institution: | Department of Microbiology, Nanjing Agricultural University, Nanjing 210095, China;Department of Microbiology, Nanjing Agricultural University, Nanjing 210095, China |
| |
| Abstract: | Summary Four mutants (DLL-E1, DLL-E2, DLL-E3 and DLL-E4) were obtained by UV and LiCl mutation breeding of Methyl Parathion degrading strain Pseudomonas sp . DLL-1. Studies on their abilities to degrade Methyl Parathion and p nitrophenol, substrates, activities of ectoenzyme and crude enzyme showed that all mutants have higher biological activities compared to the original strain. Further studies on resistance and degradation of Methyl Parathion and p nitrophenol by DLL-E4 was conducted. |
| |
| Keywords: | Methyl Parathion Pseudomonas sp Mutants Biological functions |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《土壤学报》浏览原始摘要信息 |
| 点击此处可从《土壤学报》下载免费的PDF全文 |
|
