番茄抗青枯病育种

青枯病是重庆番茄生产主要障碍之一,为选育抗病品种,在病理研究基础上,进行了抗源材料选育及利用研究。2000~2003年分离出番茄青枯病病菌68株,经致病性测定、培养性状、生化测定等研究,明确重庆市番茄青枯病病原菌为:Ralstoniasolanacearum,生理小种1,生化型Ⅲ。苗期室内人工接种抗性鉴定方法研究设伤根浸根接种法、灌注接种法、剪叶接种法三种处理,结果以伤根浸根接种法更为准确、可靠。经抗病性鉴定,结合农艺性状比较研究,筛选出四份抗源材料,并配组选出一个强优组合。

Breeding of promising tomato genotypes and hybrids against bacterial wilt

Tomato cultivation was set back in Chongqing (China) due to severe incidence of bacterial wilt caused by Ralstonia solanacearum.Hence, studies were initiated to identify the pathogen, screen resistant resources, and breed hybrids with resistance to the disease. 68 isolates of pathogen of tomato bacterial wilt were obtained during 2000-2003. The morphological characteristics, cultural characteristics, biochemical properties and pathogenicity of the isolates indicated that they were Ralstonia solanacearum,race 1, and biovar Ⅲ. Studied of methods of seedlings inoculation revealed that cutting and dipping method was the best one,compared with root-stabbing and leaf-clipping.4 tomato lines, which had high resistance to bacterial wilt, had been screened out from 184 genotypes. Among 12 hybrids that crossed from the 4 resistances, 4012 showed higher yield, good qualities, and promising resistance.