|
|||||
|
|
| 广西桂花叶枯病病原菌鉴定及其生物学特性研究 | |
| 引用本文： | 谢玲,唐晨光,岑贞陆,黄思良,秦碧霞,覃丽萍,胡春锦.广西桂花叶枯病病原菌鉴定及其生物学特性研究[J].西南农业学报,2009,22(5). |
| 作者姓名： | 谢玲 唐晨光 岑贞陆 黄思良 秦碧霞 覃丽萍 胡春锦 |
| 作者单位： | 1. 广西农业科学院微生物研究所,广西,南宁,530007 2. 长江大学农学院,湖北,荆州,434025 3. 南阳师范学院,河南,南阳,473061 4. 广西农科院植物保护研究所,广西,南宁,530007 |
| 基金项目： | 广西青年科学基金项目 |
| 摘 要： | 在广西南宁桂花树上严重发生一种叶枯病,从病叶上分离到桂花叶枯病菌(编号CHS),在致病性测定的基础上,根据病原菌株CHS的形态及ITS序列,将其鉴定为Alternaria alternata(Fr.)Keissler.对该菌的生物学特性研究结果表明:菌丝生长、孢子形成和孢子萌发的最适温度均为28 ℃;生长发育最适pH值为6～7;不同光环境对该菌菌丝生长发育影响不大,黑光环境最利于孢子萌发;在供试的培养基中PDA最适合菌丝生长和产孢;苯醚甲环唑、咪鲜胺和丙环唑对该菌的抑制作用最强.由A. alternata引起的桂花叶枯病在广西的发生属首次报道. |
| 关 键 词： | 桂花 叶枯病 鉴定 生物学特性 广西 |
Identification and Characterization of Causal Agent of Sweet Osmanthus Leaf Blight in Guangxi |
|
| XIE Ling,TANG Chen-guang,CEN Zhen-lu,HUANG Si-liang,QIN Bi-xia,QIN Li-ping,HU Chun-jin.Identification and Characterization of Causal Agent of Sweet Osmanthus Leaf Blight in Guangxi[J].Southwest China Journal of Agricultural Sciences,2009,22(5). | |
| Authors: | XIE Ling TANG Chen-guang CEN Zhen-lu HUANG Si-liang QIN Bi-xia QIN Li-ping HU Chun-jin |
| Abstract: | A sweet osmanthus leaf blight severely occurred in Nanning city, Guangxi, China. The pathogen was isolated and its pathogenicity to sweet osmanthus plants confirmed by inoculation tests. The pathogenic isolate CHS was identified as Alternaria alternata (Fr.)Keissler based on its morphological characteristics and ITS sequence. The optimum temperature and pH value for growth and conidial germination were 28 ℃ and 6-7, respectively. Different conditions of light showed no obvious effect on the mycelial growth. Black light was favorable for conidial production. Tests on fungicidal sensitivity in vitro showed that the pathogen was highly sensitive to difenoconazole, prochloraz and propiconazole. This is the first record of sweet osmanthus leaf blight caused by A. alternata in Guangxi. |
| Keywords: | Alternaria alternata rDNA-ITS |
| 本文献已被 万方数据 等数据库收录！ | |