山楂叶总黄酮三种提取方法的比较研究

以山楂品种"甜水"为试材,分别采用单因素试验法和正交试验法,研究山楂叶总黄酮3种提取方法的最佳提取工艺。结果表明:在3种提取方法中,以浸渍法提取率最高,回流提取法最低,超声波法居中;3种方法所得提取物中总黄酮含量,以回流提取法最高,超声波法与浸渍法相差不大;从总黄酮得率来看,以浸渍法所得黄酮总量最高,超声波法次之,而热回流提取法所得黄酮总量最低。但超声波法所用溶剂量最少,提取时间最短,且产率较高,是提取山楂叶总黄酮的一种非常便捷高效的方法。

Comparative Research on Three Extraction Methods of the Total Flavonoids in Hawthorn Leaves

To comparing the differences in three extraction methods of the total flavonoids in hawthorn leaves,experiments were conducted with Crataegus pinnatifida cv.'Tianshui' as materials for comparative researches on three extraction methods of the total flavonoids in hawthorn leaves.The three optimal extraction technologies were obtained by using single factor and orthogonal test design. The extraction rate,content of flavonoids and total flavonoids in three extraction methods were determined.The results showed that in three extraction methods,the extraction rate of immersion method was highest,reflux method was lowest,and supersonic method was between them;and the content of flavonoids of reflux method was highest,supersonic method was similar to immersion method;while the total flavonoids of the immersion method was highest and supersonic method followed,the lowest was reflux method.Every extraction method had its own features.The highest extraction rate and total flavonoids were obtained by immersion method with simple equipment,but longer extraction time.The highest content of flalvonoids was obtained by reflux method,which needed shorter extraction time.And in three extraction methods,supersonic extraction was a very convenient and efficient method,which needed least alcohol,shortest extraction time with higher yield.