四种AM真菌接种剂的田间效应及其分子检测研究

采用灭菌土壤生产了 4种AM真菌接种剂。在盆栽条件下测试了接种剂的质量 ,结果显示 ,4种接种剂促进玉米生长效果明显 ,地上部分生物量均显著高于对照 (p <0 .0 1 ) ;以MPN试验检测了接种剂的侵染能力 ,结果表明每克接种剂中真菌的繁殖体数在 95～ 1 4 0 0之间。将AM真菌的预接种技术和农业生产上的营养钵育苗技术相结合 ,进行了玉米的田间试验 ,结果显示 ,玉米根系的AM真菌感染率早期增长较快 ,然后趋于平稳 ;AM真菌接种剂A(Glomusconstrictum)、C (Glomus三种菌混合 )和D (G .intraradices)对玉米籽粒产量有显著的增产效果 (p <0 .0 5 ) ;玉米籽粒的淀粉含量和磷含量也高于对照。运用特异性分子探针和nest ed PCR技术 ,从田间接种AM真菌Glomusintraradices和G .mosseae的玉米根样中粗提DNA进行特异性扩增 ,成功地从感染根段中检测到特定的接种AM真菌。本工作从分子水平为评价高效AM真菌的应用潜力、研究AM真菌之间及其与其他微生物之间的相互关系奠定了基础。

MOLECULAR DETECTION OF FOUR ARBUSCULAR MYCORRHIZAL FUNGAL INOCULA IN FIELD TRIALS

Four arbuscular mycorrhizal fungi (AMF) inocula were produced in the sterilized soil under glasshouse conditions.Effectiveness and infectivity of the inocula were tested in pot culture with the most probable number (MPN) test method.The results indicated that biomass of inoculated plants was significantly higher than that of non-inoculated control plants (p<0.01),and the number of propagules was in the range of 95~1 400 per gram inocula.The field trials were carried out by means of producing pre-inoculated seedlings prior to transplanting.The infection rate of maize roots increased rapidly at early stage of the plant growth and then leveled off.The yield of the maize inoculated with inoculum A (Glomus constrictum),C (three Glomus species) or D (G.intraradices) was significantly enhanced (p< 0.05),and so was the content of starch and phosphorus in grains of the maize.