| 灵芝多糖含量检测条件及其提取工艺的优化 |
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| 引用本文： | 王新.灵芝多糖含量检测条件及其提取工艺的优化[J].安徽农业科学,2007,35(14):4281-4282,4285. |
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| 作者姓名： | 王新 |
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| 作者单位： | 江苏大学食品与生物工程学院,江苏镇江,212013 |
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| 摘 要： | 优化了苯酚-硫酸法测定灵芝多糖含量的显色条件,并且通过单因素和正交试验确定了灵芝多糖的最佳提取工艺条件。结果表明：苯酚-硫酸法测定灵芝多糖含量的条件为最大吸收波长490 nm,显色时间20 min,显色温度100℃;灵芝多糖的最佳提取工艺条件为提取温度80℃,提取时间2.5 h,液料比50 ml/g,在此条件下灵芝多糖的提取率为2.58%。
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| 关 键 词： | 苯酚-硫酸法 灵芝多糖 正交试验 |
| 文章编号： | 0517-6611（2007）14-04281-02 |
| 收稿时间： | 2007-02-07 |
| 修稿时间： | 2007-02-07 |
Optimization of Polysaccharides Content Decection condition and Extraction Process of Ganoderma lucidum |
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| WANG Xin.Optimization of Polysaccharides Content Decection condition and Extraction Process of Ganoderma lucidum[J].Journal of Anhui Agricultural Sciences,2007,35(14):4281-4282,4285. |
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| Authors: | WANG Xin |
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| Institution: | School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013 |
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| Abstract: | The color condition for detecting polysaccharides in Ganoderma lucidum by phenol-sulferic acid method was optimized.The optimum extracting condition was obtained through single effect factor and orthogonal experiments.The condition of phenol-sulferic acid method was 490 nm maximum absorption wavelength,20 min reaction time and 100 ℃ reaction temperature.The optimum extracting condition was 50 minutes,60 ℃ and 50 ml/g liquid-material ratio.Extraction ratio of polysaccharides under the above condition was 2.58 %. |
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| Keywords: | Phenol-sulferic acid method Polysaccharides in Ganoderma lucidum Orthogonal experiment |
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