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| 利用SCAR标记鉴定'陇椒5号'辣椒种子的纯度 | |
| 引用本文： | 陈灵芝,张建农,王兰兰,魏兵强. 利用SCAR标记鉴定'陇椒5号'辣椒种子的纯度[J]. 甘肃农业大学学报, 2011, 46(1): 55-57 |
| 作者姓名： | 陈灵芝 张建农 王兰兰 魏兵强 |
| 作者单位： | 1. 甘肃农业大学农学院,甘肃,兰州,730070;甘肃省农业科学院蔬菜研究所,甘肃,兰州,730070 2. 甘肃农业大学农学院,甘肃,兰州,730070 3. 甘肃省农业科学院蔬菜研究所,甘肃,兰州,730070 |
| 摘 要： | 以‘陇椒5号'辣椒及其父、母本为试材,提取基因组DNA,利用SCAR标记鉴定其种子纯度.结果表明:从辣椒多态性丰富的150条引物中筛选获得1条可用于鉴别母本和F1的引物RG520,1条可用于鉴别父本和F1的引物RG780;将R520进行克隆、测序,设计出1对SCAR引物,能特异鉴别F1和母本. |
| 关 键 词： | 辣椒 SCAR标记 纯度鉴定 |
Identification of genetic purity of pepper of 'Longjiao No.5' by SCAR |
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| CHEN Ling-zhi,ZHANG Jian-nong,WANG Lan-lan,WEI Bing-qiang. Identification of genetic purity of pepper of 'Longjiao No.5' by SCAR[J]. Journal of Gansu Agricultural University, 2011, 46(1): 55-57 | |
| Authors: | CHEN Ling-zhi ZHANG Jian-nong WANG Lan-lan WEI Bing-qiang |
| Abstract: | Genetic purity of pepper of 'Longjiao No.5' was identified by DNA extraction and SCAR with this cultivar and its parents as materials.The results showed that the primers of RG520 and RG780 could be used for separating female and male parent from F1 hybrid from 150 arbitary oligonucheotide primers.The band of RG520 was cloned and sequenced to be designed as a SCAR marker for separating female parent from F1 hybrid. |
| Keywords: | pepper SCAR marker purity identification |
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