| 基本培养基及激素和附加物对黄芪组织培养的影响 |
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| 引用本文： | 张延红,陈红刚,高素芳,晋玲. 基本培养基及激素和附加物对黄芪组织培养的影响[J]. 甘肃农业科技, 2011, 0(2): 13-15 |
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| 作者姓名： | 张延红 陈红刚 高素芳 晋玲 |
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| 作者单位： | 甘肃中医学院药学系,甘肃,兰州,730000 |
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| 基金项目： | 十一五国家科技攻关西部支撑项目当归、黄芪、大黄种质资源可持续利用关键技术研究,甘肃省教育厅项目甘肃道地黄芪工厂化育苗技术研究,甘肃中医学院教改项目药用植物组织培养技术实验内容的创新研究 |
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| 摘 要： | 黄芪茎段组织培养研究的结果表明,黄芪茎段的最佳初代培养基为MS+6-BA 0.1 mg/L和MS+6-BA2.5 mg/L+NAA0.5 mg/L;MS+6-BA2.5mg/L+NAA 0.5 mg/L可作为继代培养基;高浓度的6-BA可促进黄芪短枝产生不定芽;附加物对短枝生长的影响随浓度和种类而变化,但促进生长的影响...
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| 关 键 词： | 蒙古黄芪 组织培养 培养基 激素 附加物 |
Effects of Basic Medium,Hormone and Addendum on Tissue Culture of Astragalus membranaceus Bge. |
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| ZHANG Yan-hong,CHEN Hong-gang,GAO Su-fang,JIN Ling. Effects of Basic Medium,Hormone and Addendum on Tissue Culture of Astragalus membranaceus Bge.[J]. Gansu Agricultural Science and Technology, 2011, 0(2): 13-15 |
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| Authors: | ZHANG Yan-hong CHEN Hong-gang GAO Su-fang JIN Ling |
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| Affiliation: | ZHANG Yan-hong,CHEN Hong-gang,GAO Su-fang,JIN Ling(Department of Herbs,Gansu College of TCM,Lanzhou Gansu 730000,China) |
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| Abstract: | The effects of basic medium type,hormone and addendum on tissue culture of Astragalus membranaceus Bge.was studies.The results showed that the optimum medium for primary is MS + 6-BA 0.1 mg/L and MS + 6-BA 2.5 mg/L + NAA 0.5 mg/L;the optimum medium for subculture is MS + 6-BA 2.5 mg/L + NAA0.5 mg/L;high concentration of 6-BA can promote the induction of adventitious buds;the addendum has not obviously influence of shoot growth.However,the promote growth had no significant influence. |
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| Keywords: | Astragalus membranaceus Bge. Tissue culture Medium Hormone Addendum |
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